PMID: 58946Jan 1, 1976

Antigen and antibody purification by immunoadsorption: elimination of non-biospecifically bound proteins

Journal of Immunological Methods
M Zoller, S Matzku

Abstract

Immunoadsorption is a simple and effective method for purifying antigens and antibodies. The major interfering effect is non-biospecific binding, especially when crude preparations of antibodies or antigens are coupled to the matrix. In this paper various possibilities for eliminating these effects are compared and analysed. It was found that for antigen (AFP) purification pre-elution with a pH 10.0 buffer of high ionic strength (1.0 M) is effective. A major problem in purifying antibodies in the non-biospecific binding of IgG. This can be eliminated by using a non-biospecific immunoadsorbent column, where specific antibodies are not, or only weakly bound and IgG with irrelevant specificity but exhibiting high non-biospecific interaction is retained. This has been applied to preparation of sheep anti-pepsinogen II group protease antibodies.

References

Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Shaltiel, Z Er-El
Oct 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·P CuatrecasasC B Anfinsen
Aug 1, 1971·Die Naturwissenschaften·G Feinstein

Citations

Jun 15, 1979·Molecular and Cellular Biochemistry·M Czokało
Mar 15, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·G R CannellM J Thomas
May 1, 1977·Immunochemistry·S Matzku, M Zöller
Oct 10, 1985·Journal of Immunological Methods·I Cayzer
May 12, 1989·Journal of Immunological Methods·D R DannhornC Kirchner
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J RejmanW L Hurley
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R S Hansen, J A Beavo
Jul 1, 1978·Analytical Biochemistry·J A SmithS J Leach
Feb 1, 1988·European Journal of Clinical Investigation·A GustafsonR J Havel
Dec 3, 2014·Journal of Proteomics·Dimitrios KorbakisEleftherios P Diamandis
Jan 1, 1976·Transplantation Reviews·R W Baldwin
Jan 1, 1981·Hypertension·M IkedaJ Genest
Mar 15, 1977·FEBS Letters·J M RenoirC Mercier-Bodard
Mar 17, 2004·Oncogene·Nesrine G TarbéUlrich H Weidle
Jun 15, 1979·International Journal of Cancer. Journal International Du Cancer·J G Bowen, R W Baldwin
Dec 15, 1977·International Journal of Cancer. Journal International Du Cancer·M J Lopez, D M Thomson

Related Concepts

Process of Absorption
Alpha-Fetoproteins
Antigens
Chromatography, Affinity
Fetal Proteins
Immunoassay Method
Pepsinogen B
Plasma Protein Binding Capacity
Sepharose

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