Antigen-binding sites dominate the surface properties of IgG antibodies

Molecular Immunology
C WingrenM Ohlin

Abstract

A new technique, liquid-liquid partition chromatography in an aqueous polyethylene glycol-dextran two-phase system, was used to detect differences in surface properties of antibodies with different antigen-binding sites. Employing well-characterized monoclonal IgG antibodies and Fab and Fc fragments thereof as well as chimeric IgG antibodies we found a remarkable relationship between structure of the antibody combining site and chromatographic behaviour. The surface properties of the IgG antibodies were dominated by those of its antigen-binding regions. In addition, our results indicated that the constant parts of the IgGs form similar scaffoldings, on to which CDRs of variable shapes and sizes are interspaced and constitute the major dominant differences in exposed surface properties.

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J SchlessingerI Pecht
Jan 1, 1992·Advances in Immunology·D R Burton, J M Woof
Jan 1, 1992·Molecular Immunology·J LundG Winter
Jun 20, 1990·European Journal of Biochemistry·S RyazantsevV Abramov
Dec 1, 1991·Molecular Immunology·R J Poljak
Dec 1, 1991·Molecular Immunology·D Givol
Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M S ChappelM H Klein
Apr 1, 1991·Biochemical Society Transactions·I D Campbell
Aug 15, 1991·Analytical Biochemistry·H WalterD E Brooks
Feb 22, 1990·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C Milstein
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L K TanS L Morrison
Jul 1, 1990·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G LiljaS G Johansson
Jul 1, 1985·Scandinavian Journal of Immunology·U B HanssonF D Lindström
Dec 21, 1989·Nature·C ChothiaW R Tulip
Jan 1, 1988·Advances in Immunology·E D GetzoffH M Geysen
Apr 21, 1988·Nature·A R Duncan, G Winter
Jan 1, 1988·Molecular Immunology·D M YarmushM L Yarmush
Nov 1, 1987·The Journal of Experimental Medicine·M BrüggemannM S Neuberger
Jul 1, 1983·Journal of Steroid Biochemistry·R D FarrantJ K Sanders
May 1, 1995·The Journal of Allergy and Clinical Immunology·C G Magnusson
May 11, 1995·Biochimica Et Biophysica Acta·C Wingren, U B Hansson
Feb 1, 1994·Molecular Immunology·E A Padlan
Feb 1, 1993·Current Opinion in Immunology·R A Mariuzza, R J Poljak
May 1, 1993·European Journal of Immunology·J GreenwoodH Waldmann
Jan 1, 1962·Progress in Allergy·O OUCHTERLONY
Jun 1, 1993·Avian Pathology : Journal of the W.V.P.A·D A Higgins, G W Warr

❮ Previous
Next ❯

Citations

May 17, 1996·Journal of Chromatography. B, Biomedical Applications·C Wingren, U B Hansson
Jun 20, 1997·Biochimica Et Biophysica Acta·U B HanssonU Alkner
Oct 1, 1995·Scandinavian Journal of Immunology·K AnderssonU Alkner
Jul 10, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Adel A A Ismail
Jun 19, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hye-Mee ParkYoon-Mo Koo
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.