Antigen capture ELISA system for henipaviruses using polyclonal antibodies obtained by DNA immunization.

Archives of Virology
Yoshihiro KakuLin-Fa Wang

Abstract

A novel antigen-capture sandwich ELISA system targeting the glycoproteins of the henipaviruses Nipah virus (NiV) and Hendra virus (HeV) was developed. Utilizing purified polyclonal antibodies derived from NiV glycoprotein-encoding DNA-immunized rabbits, we established a system that can detect the native antigenic structures of the henipavirus surface glycoproteins using simplified and inexpensive methods. The lowest detection limit against live viruses was achieved for NiV Bangladesh strain, 2.5 × 10(4) TCID(50). Considering the recent emergence of genetic variants of henipaviruses and the resultant problems that arise for PCR-based detection, this system could serve as an alternative rapid diagnostic and detection assay.

References

May 4, 2001·Microbes and Infection·P DanielsB T Eaton
Sep 7, 2001·Journal of Virological Methods·I L SmithG A Smith
Mar 5, 2003·Antiviral Research·Sai-Kit Lam
Dec 17, 2005·Nature Reviews. Microbiology·Bryan T EatonLin-Fa Wang
Jan 31, 2006·Expert Review of Anti-infective Therapy·Katharine N Bossart, Christopher C Broder
Dec 3, 2008·Emerging Infectious Diseases·Yan LiZhengli Shi
May 30, 2009·Journal of Virological Methods·K S FeldmanL-F Wang
Jul 29, 2009·PloS One·Jan Felix DrexlerChristian Drosten
Dec 8, 2009·Thrombosis and Haemostasis·Andrea MaisnerHana Weingartl
Jun 8, 2010·Virology Journal·Cheng-Feng ChiangPierre E Rollin
Oct 30, 2010·Emerging Infectious Diseases·Glenn A MarshLin-Fa Wang
Oct 8, 2011·PloS One·Ina SmithHume Field
Feb 7, 2012·Emerging Infectious Diseases·Michael K LoPaul A Rota

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Citations

Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·D D KulkarniD Senthil Kumar
Mar 29, 2013·Future Microbiology·Briony HazeltonDominic E Dwyer
Mar 1, 2019·BMJ Global Health·Laura T Mazzola, Cassandra Kelly-Cirino
Aug 16, 2015·Brain Pathology·Kien Chai Ong, Kum Thong Wong

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