Antigen delivery with poly(propylacrylic acid) conjugation enhances MHC-1 presentation and T-cell activation.

Bioconjugate Chemistry
Suzanne FlanaryPatrick S Stayton

Abstract

While many infectious diseases are controlled by vaccine strategies, important limitations continue to motivate the development of better antigen delivery systems. This study focuses on the use of a pH-sensitive polymeric carrier based on poly(propylacrylic acid) (PPAA) to address the need for more potent CD8 cytotoxic T-cell (CTL) responses. An MHC-1/CD8 CTL cell model system with ovalbumin as the protein antigen was used to test whether PPAA could enhance the delivery of ovalbumin into the MHC-1 display pathway. Ovalbumin was conjugated to poly(propylacrylic acid-co-pyridyldisulfide acrylate) (PPAA-PDSA) by disulfide exchange to make reversible conjugates that could be reduced by the glutathione redox system in the cytosol of antigen presenting cells. The PPAA-PDSA ovalbumin conjugates displayed the pH-sensitive membrane disruptive properties of the parent polymer as determined by their hemolysis activities (sharply active at the endosomal pH values of 6-6.5). The polymer-ovalbumin conjugates exhibited strong 22-fold increases in the MHC-1 presentation and ovalbumin-specific CTL activation compared to free ovalbumin. No CTL activation was observed with control conjugates of ovalbumin and poly(methylacrylic acid) (PMAA) that d...Continue Reading

References

Jan 1, 1987·Annual Review of Biochemistry·D C Wiley, J J Skehel
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Jan 1, 1994·Annual Review of Immunology·T BoonA Van Pel
Mar 1, 1994·International Immunology·S Sanderson, N Shastri
Jul 1, 1997·Physiological Reviews·S MukherjeeF R Maxfield
May 1, 1997·Human Immunology·T J GoletzJ A Berzofsky
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·T J GoletzJ A Berzofsky
Jun 22, 1999·Infection and Immunity·A M DolingM N Starnbach
Sep 2, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·N MurthyA S Hoffman
Mar 4, 2000·Advanced Drug Delivery Reviews·R WattiauxM Jadot
Apr 11, 2000·Journal of Biomedical Materials Research·K D NewmanJ Samuel
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Feb 13, 2001·Vaccine·P Moingeon
Nov 22, 2001·Bioconjugate Chemistry·C Y CheungA S Hoffman
Feb 28, 2002·Annual Review of Immunology·Pierre GuermonprezSebastian Amigorena
Aug 3, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jayanth PanyamVinod Labhasetwar
Mar 20, 2003·Bioconjugate Chemistry·Niren MurthyPatrick S Stayton
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Niren MurthyJean M J Fréchet
Nov 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Volga BulmusAllan Hoffman
Apr 7, 2004·Advanced Drug Delivery Reviews·Véronique Cabiaux
Nov 16, 2004·The Journal of Gene Medicine·Akin AkincRobert Langer
Mar 15, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Eun Seong LeeYou Han Bae
Apr 12, 2005·Protein Engineering, Design & Selection : PEDS·Brian AlbarranPatrick S Stayton
Jun 7, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Young Jik KwonJean M J Fréchet
Jun 29, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohamed E H El-SayedPatrick S Stayton
Jul 13, 2005·Cancer Immunology, Immunotherapy : CII·K L Knutson, M L Disis
Sep 27, 2005·Immunological Reviews·Kenneth L Rock, Lianjun Shen
Nov 17, 2005·Bioconjugate Chemistry·Michael J Heffernan, Niren Murthy
Aug 22, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kyung T OhAlexander V Kabanov
Aug 22, 2006·Immunobiology·Paul J TackenCarl G Figdor

❮ Previous
Next ❯

Citations

Oct 19, 2012·Molecular Pharmaceutics·Geoffrey Y BerguigPatrick S Stayton
Oct 31, 2013·BioMed Research International·Austin B GardnerAbhinav P Acharya
Jul 25, 2012·Therapeutic Delivery·Donghyun HongDongwon Lee
Apr 5, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Salka KellerPatrick S Stayton
May 23, 2014·Pharmaceutical Research·Preety SahdevJames J Moon
Jun 28, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Emily CrownoverPatrick S Stayton
Aug 6, 2011·Macromolecules·Jiao LuMolly S Shoichet
Jan 23, 2013·Current Opinion in Immunology·Weiwei Aileen Li, David J Mooney
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Motao ZhuGuangjun Nie
Feb 19, 2015·Therapeutic Delivery·Nishi ModySuresh P Vyas
May 25, 2016·Expert Opinion on Drug Delivery·Mahdi KarimiMichael R Hamblin
Aug 24, 2016·Molecular Pharmaceutics·Chintan H KapadiaJoseph M DeSimone
Jul 30, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Flavia FontanaHélder A Santos
Nov 27, 2009·Nature·Jeffrey A HubbellMelody A Swartz
May 30, 2012·Advanced Materials·James J MoonDarrell J Irvine
Jan 23, 2019·Advanced Healthcare Materials·Eun Sook LeeJae Hyung Park
Oct 26, 2012·Current Opinion in Allergy and Clinical Immunology·Emanuela Fabiola Craparo, Maria Luisa Bondì
Apr 25, 2012·Annual Review of Biomedical Engineering·Kim A WoodrowDavid D Lo
Jan 21, 2020·Archives of Pharmacal Research·Md AsadujjamanJun-Pil Jee
Aug 11, 2020·International Journal of Pharmaceutics: X·Marie GarinotJean Haensler
Jun 3, 2021·Vaccines·Natassa PippaStergios Pispas
Feb 13, 2017·ACS Biomaterials Science & Engineering·Charles B ChessonJai S Rudra

❮ Previous
Next ❯

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.