PMID: 7009753Jan 1, 1980Paper

Antigen density in ELISA; effect on avidity dependency

Journal of Immunological Methods
O P Lehtonen, M K Viljanen

Abstract

Two different densities of an antigen (bovine serum albumin, BSA) were used on paper disks is an enzyme-linked immunosorbent assay (ELISA) for chicken IgG anti-BSA antibodies. Disks of different antigen density but containing the same amount of the antigen showed a difference in immunoreactivity. Samples containing large amounts of low avidity antibodies gave higher absorbance when disks of lower antigen density were used. This phenomenon was not due to a competition between antibodies of various immunoglobulin classes. These findings may indicate that antigen density affects the sensitivity of ELISA to antibodies of various avidities.

References

Oct 1, 1972·Immunochemistry·M Ceska, U Lundkvist
Mar 1, 1969·Journal of Biomedical Materials Research·J L Brash, D J Lyman
Jan 1, 1980·Journal of Immunological Methods·O P Lehtonen, M K Viljanen

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Citations

Aug 18, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·E F WakefieldC S Hosking
Oct 29, 1982·Journal of Immunological Methods·O P Lehtonen, E Eerola
May 11, 1984·Journal of Immunological Methods·J P Rotmans, B A Scheven
Apr 22, 1985·Journal of Immunological Methods·M W Steward, A M Lew
Dec 17, 1985·Journal of Immunological Methods·M LiebertT R Hakala
Jun 26, 1987·Journal of Immunological Methods·J D BeattyW G Vlahos
Jul 27, 2011·Colloids and Surfaces. B, Biointerfaces·Ping PengGil Garnier
Dec 1, 1986·Applied and Environmental Microbiology·C EdwinS K Maheswaran
Jul 1, 1986·Immunology Today·S Ghosh, A M Campbell

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