Antigen detection and apoptosis in Mongolian gerbil's kidney experimentally intraperitoneally infected by swine hepatitis E virus

Virus Research
Majid Hussain SoomroHeng Li

Abstract

We examined the effect of hepatitis E virus (HEV) on the renal tissue pathogenesis, morphological damages and related molecular mechanisms following swine HEV suspension intraperitoneally inoculation in Mongolian gerbils. The microscopic and ultramicroscopic analyses of kidney tissue structure were carried out at different points after inoculation of HEV. The immunohistochemistry, real-time PCR and Western blot were performed to explore the molecular mechanisms associated with HEV presence in the renal tissues. Real-time PCR revealed that the copies of HEV RNA in the kidney were detected at 7 dpi, and peaked at 14 dpi at a concentration was 7.18 logs g(-1), with detection of HEV ORF2 antigen by immunohistochemistry. Hematoxylin and eosin (HE) staining showed pathological lesions including glomerular atrophy, degeneration, edema and necrosis of renal tubular epithelial cells and Mallory and Sirius red staining indicated the presence of collagen fibers and fibrosis in kidney tissues of inoculated gerbils. Ultrastructural studies of basal membrane of renal tubules demonstrated the rough and uneven with mitochondria swelling and vacuolation in the tissues of HEV inoculated animals. Similarly, significantly higher number of (TUNEL)-...Continue Reading

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Citations

Jan 30, 2020·Journal of Clinical Medicine·Sébastien LhommeNassim Kamar
May 5, 2017·Food and Environmental Virology·Nigel CookReimar Johne
Nov 14, 2018·Nature Communications·Jordi GouillyHicham El Costa
Apr 6, 2019·Frontiers in Cellular and Infection Microbiology·Jijing TianMajid Hussain Soomro
Jul 10, 2019·Viruses·Thomas HorvatitsSven Pischke
Jul 11, 2017·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Rosina Girones
Oct 20, 2018·Frontiers in Microbiology·Tianlong LiuRuihan Shi
Jan 22, 2021·Reviews in Medical Virology·Mohamed A El-Mokhtar, Ibrahim M Sayed
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Jan 20, 2019·The Journal of Infection·Olivier MarionJacques Izopet
Mar 2, 2022·Microbiology Spectrum·Ling-Dong XuYao-Wei Huang

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