Antigen detection as a point-of-care test for TB: the case of lipoarabinomannan

Future Microbiology
Soumya Swaminathan, V V Banu Rekha

Abstract

The limitations of sputum smear microscopy, routine chest radiology for HIV-associated TB and culture-based diagnosis are well recognized, especially in resource-limited settings. The diagnostic accuracy of a new point-of-care lateral-flow urine strip test for lipoarabinomannan (Determine(®) TB-LAM; Alere, MA, USA), which costs US$3.50 per test strip and provides results within 30 min, was evaluated in a cohort of South African patients for HIV-associated TB before starting anti-retroviral therapy in South Africa. Prevalence of culture-positive TB cases was 17.4%, among which 28.2% had sputum smear positivity. Determine(®) TB-LAM (Alere, MA, USA) had highest sensitivity at low CD4 cell counts: 66.7, 51.7 and 39.0% at <50 cells, <100 cells and <200 cells per µl, respectively; specificity was greater than 98% for all strata. There was an incremental sensitivity when Determine TB-LAM was combined with smear microscopy, which did not differ statistically from the sensitivities obtained by testing a single sputum sample with the Xpert(®) MTB/RIF (Cepheid; CA, USA) assay. Determine TB-LAM is a simple, low-cost alternative to existing diagnostic assays for TB screening in HIV-infected patients with very low CD4(+) cell counts.

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Citations

May 2, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Gompol SuwanpimolkulKiat Ruxrungtham
Jan 23, 2014·The Cochrane Database of Systematic Reviews·Karen R SteingartNandini Dendukuri
May 11, 2016·The Cochrane Database of Systematic Reviews·Maunank ShahKaren R Steingart
Feb 19, 2019·ERJ Open Research·Margarida Correia-NevesGunilla Källenius

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Methods Mentioned

BETA
ELISA

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