Antigen Discovery, Bioinformatics and Biological Characterization of Novel Immunodominant Babesia microti Antigens.

Scientific Reports
Nitin VermaSanjai Kumar

Abstract

Babesia microti is an intraerythrocytic parasite and the primary causative agent of human babesiosis. It is transmitted by Ixodes ticks, transfusion of blood and blood products, organ donation, and perinatally. Despite its global public health impact, limited progress has been made to identify and characterize immunodominant B. microti antigens for diagnostic and vaccine use. Using genome-wide immunoscreening, we identified 56 B. microti antigens, including some previously uncharacterized antigens. Thirty of the most immunodominant B. microti antigens were expressed as recombinant proteins in E. coli. Among these, the combined use of two novel antigens and one previously described antigen provided 96% sensitivity and 100% specificity in identifying B. microti antibody containing sera in an ELISA. Using extensive computational sequence and bioinformatics analyses and cellular localization studies, we have clarified the domain architectures, potential biological functions, and evolutionary relationships of the most immunodominant B. microti antigens. Notably, we found that the BMN-family antigens are not monophyletic as currently annotated, but rather can be categorized into two evolutionary unrelated groups of BMN proteins respe...Continue Reading

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Citations

Sep 6, 2020·Current Opinion in Hematology·Cheryl A LoboMarilis Rodriguez
Feb 5, 2021·Acta Biochimica Et Biophysica Sinica·Huan QiSheng-Ce Tao
May 15, 2021·Cell Host & Microbe·Hanan A Al-NazalMichael F Good

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Datasets Mentioned

BETA
BAC07532.1

Methods Mentioned

BETA
Fluorescence
PCR
phage display
enzyme-linked immunosorbent
antibody assay
phage library
phage
ELISA
antibody
electrophoresis

Software Mentioned

FastTree
TMHMM
BLASTCLUST
Muscle
MacVector
BLAST
SignalP
Kalign2
Imaris
Huygens

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Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.