PMID: 6971903Jan 1, 1981Paper

Antigen-induced proliferation of murine T-lymphocytes in vitro. II. The effect of different macrophage populations on the antigen-induced proliferative response

Journal of Immunological Methods
S Z Ben-Sasson, J Kagan

Abstract

Various macrophage-containing preparations were tested for their ability to increase the antigen-specific proliferative response of murine T-lymphocytes. The preparations examined included: peritoneal exudate cells (PEC) from mice injected with mineral oil or thioglycolate; fresh bone-marrow cells; bone marrow cells grown in culture for up to 11 days; normal spleen cells, and spleen cells from mice injected with mineral oil. The best proliferative response was obtained when the lymphocytes were supplemented with 30% spleen cells from mice injected with mineral oil. When spleen cells from mineral oil injected mice are compared with those of spleen cells from normal mice, it is evident that mineral oil given i.p. activates the spleen macrophages. Although the number and percentage of macrophages in the spleen does not increase following mineral oil injection, the activities of some of their enzymes (acid phosphatase and beta-glucuronidase) increase while others do not change (Cathepsin D and lysozyme). Furthermore, the Fc-dependent phagocytic activity of spleen macrophages and their spreading on plastic culture dishes is increased after mineral oil treatment. We conclude that the activation of spleen macrophages caused by an i.p....Continue Reading

References

Jul 1, 1978·European Journal of Immunology·G S HansenA Svejgaard
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R M Steinman, M D Witmer
Jan 1, 1978·Immunological Reviews·E R Unanue
Jan 1, 1979·The Journal of Experimental Medicine·J W van der MeerR van Furth
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Nov 1, 1968·Archives of Biochemistry and Biophysics·C BeckA L Tappel
Mar 1, 1980·European Journal of Immunology·P L Mottram, J F Miller
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C Y LuE R Unanue

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Citations

Mar 11, 1991·Immunology Today·F D FinkelmanA Sher
Feb 6, 2004·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Leman YelSudhir Gupta
May 1, 1987·Pediatric Annals·I Berkower, H Z Streicher

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