Antigen-Mediated, Macrophage-Stimulated, Accelerated Wound Healing Using α-Gal Nanoparticles

Annals of Plastic Surgery
Omer E KaymakcalanJason A Spector

Abstract

Macrophages are known to be crucial to timely and efficacious wound healing. They have been shown to modulate inflammation and the migration and proliferation of regenerative cells, promoting tissue deposition and wound closure. This study explored the use of the natural antigen Galα1-3Galβ1-4GlcNAc-R (α-gal), present in lower mammals yet absent in Old World primates and humans, to induce a transiently enhanced macrophage response and thereby direct accelerated wound closure and healing in a standard murine model. α1,3galactosyltransferase knockout mice were stimulated to produce anti-Gal antibodies at levels comparable with humans. α-Gal-containing micelle nanoparticles were generated and applied to full-thickness splinted wounds on the mice. At 1, 2, 3, 6, and 9 days postoperatively, mice were killed, and wounds were analyzed histologically for macrophage invasion, epithelialization, vascularization, and granulation tissue deposition. Flow cytometry of wound tissue was performed to quantify relative levels of proinflammatory M1 to anti-inflammatory M2 macrophage subtypes. Treatment of splinted full-thickness murine wounds with α-gal-containing nanoparticles led to accelerated wound healing and closure as demonstrated by accel...Continue Reading

References

Jan 1, 1984·Annual Review of Immunology·R Snyderman, M C Pike
Nov 1, 1984·The Journal of Experimental Medicine·U GaliliI Flechner
Mar 25, 1998·The Journal of Antimicrobial Chemotherapy·M R Haeney
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Robert D GalianoGeoffrey C Gurtner
Jan 13, 2005·JAMA : the Journal of the American Medical Association·Nalini SinghBenjamin A Lipsky
Jun 9, 2009·Burns : Journal of the International Society for Burn Injuries·Uri GaliliUssama M Abdel-Motal
Oct 24, 2009·The American Journal of Pathology·Rita MirzaTimothy J Koh
Nov 12, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K SenMichael T Longaker
Sep 18, 2010·Journal of the American Podiatric Medical Association·Vickie R DriverGary Gibbons
Sep 22, 2010·Nature Immunology·Subhra K Biswas, Alberto Mantovani
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kim M WigglesworthUri Galili
Nov 29, 2011·Dermatologic Clinics·Alina Markova, Eliot N Mostow
Jan 31, 2012·Plastic and Reconstructive Surgery·Zachary M HurwitzUri Galili
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Feb 22, 2013·Cancer Imaging : the Official Publication of the International Cancer Imaging Society·Stephan K HaerleDaniel T Schmid
Jun 6, 2013·Cancer Biomarkers : Section a of Disease Markers·Xiaomu ZhaoZhongtao Zhang
Oct 2, 2013·Immunobiology·Bénédicte Chazaud
Mar 4, 2014·Advances in Wound Care·Anca Sindrilaru, Karin Scharffetter-Kochanek
Feb 14, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shin-ichiro OkizakiMasataka Majima
Mar 1, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Swapna GhantaBabak J Mehrara
Apr 15, 2015·The American Journal of Pathology·Ermelindo C LealAristidis Veves
Apr 16, 2015·The Journal of Pathology·Rita E MirzaTimothy J Koh
Sep 5, 2015·Advances in Wound Care·Robert G Frykberg, Jaminelli Banks
Nov 7, 2015·Advances in Wound Care·Peter Adam ReesArdeshir Bayat
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ming GaoMayland Chang
Jan 6, 2017·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·Kerstin KlinkertNoel M Caplice

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Citations

Feb 7, 2020·Experimental Dermatology·Omer E KaymakcalanJason A Spector
Jul 18, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Menglei WangLi Li

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