PMID: 2121888Nov 1, 1990Paper

Antigen processing by epidermal Langerhans cells correlates with the level of biosynthesis of major histocompatibility complex class II molecules and expression of invariant chain

The Journal of Experimental Medicine
E PuréR M Steinman

Abstract

Two prior studies with a small number of T cell lines have shown that the presentation of native protein antigens by epidermal Langerhans cells (LC) is regulated. When freshly isolated, LC are efficient antigen-presenting cells (APC), but after a period of culture LC are inefficient or even inactive. The deficit in culture seems to be a selective loss in antigen processing, since cultured LC are otherwise rich in major histocompatibility complex (MHC) class II products and are active APC for alloantigens and mitogens, which do not require processing. We have extended the analysis by studying presentation to bulk populations of primed lymph node and a T-T hybrid. Only freshly isolated LC can be pulsed with the protein antigens myoglobin and conalbumin, but once pulsed, antigen is retained in an immunogenic form for at least 2 d. The acquisition of antigen, presumably as MHC-peptide complexes, is inhibited if the fresh LC are exposed to foreign protein in the presence of chloroquine or cycloheximide. The latter, in contrast, improves the efficacy of antigen pulsing in anti-Ig-stimulated B blasts. In additional studies of mechanism, we noted that both fresh and cultured LC endocytose similar amounts of an antigen, rhodamineovalbum...Continue Reading

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Citations

Dec 8, 1998·European Journal of Immunology·B CondaminetO Berthier-Vergnes
Mar 1, 1996·Stem Cells·R Thomas, P E Lipsky
Dec 4, 1998·Springer Seminars in Immunopathology·R Thomas
Jan 1, 1992·Cancer Immunology, Immunotherapy : CII·J P ZouT Hamaoka
Feb 1, 1996·Medical Microbiology and Immunology·H MollC Blank
Jan 1, 1994·Molecular Aspects of Medicine·A MorrisS Young
Jun 1, 1991·Current Opinion in Immunology·R M Steinman, J W Young
Jun 15, 2005·Seminars in Immunology·Jenny Valladeau, Sem Saeland
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Nov 9, 2000·Veterinary Immunology and Immunopathology·C J Howard, J C Hope
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Aug 1, 1993·The Journal of Experimental Medicine·C Reis e SousaJ M Austyn

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