Antigen receptor editing in anti-DNA transitional B cells deficient for surface IgM

The Journal of Immunology : Official Journal of the American Association of Immunologists
Kerstin KieferMelvin J Bosma

Abstract

In response to encounter with self-Ag, autoreactive B cells may undergo secondary L chain gene rearrangement (receptor editing) and change the specificity of their Ag receptor. Knowing at what differentiative stage(s) developing B cells undergo receptor editing is important for understanding how self-reactive B cells are regulated. In this study, in mice with Ig transgenes coding for anti-self (DNA) Ab, we report dsDNA breaks indicative of ongoing secondary L chain rearrangement not only in bone marrow cells with a pre-B/B cell phenotype but also in immature/transitional splenic B cells with little or no surface IgM (sIgM(-/low)). L chain-edited transgenic B cells were detectable in spleen but not bone marrow and were still found to produce Ab specific for DNA (and apoptotic cells), albeit with lower affinity for DNA than the unedited transgenic Ab. We conclude that L chain editing in anti-DNA-transgenic B cells is not only ongoing in bone marrow but also in spleen. Indeed, transfer of sIgM(-/low) anti-DNA splenic B cells into SCID mice resulted in the appearance of a L chain editor (Vlambdax) in the serum of engrafted recipients. Finally, we also report evidence for ongoing L chain editing in sIgM(low) transitional splenic B c...Continue Reading

References

Apr 1, 1991·European Journal of Immunology·P SanchezP A Cazenave
Jun 1, 1991·The Journal of Experimental Medicine·H GuI Förster
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M W MooreE Selsing
Feb 10, 1983·Nature·G C BosmaM J Bosma
Dec 1, 1995·The Journal of Experimental Medicine·L A Casciola-RosenA Rosen
Oct 1, 1995·Genes & Development·D A Ramsden, M Gellert
Aug 1, 1995·The Journal of Experimental Medicine·E L Prak, M Weigert
Jan 19, 1995·Nature·C ChenM Weigert
Dec 1, 1993·The Journal of Experimental Medicine·D B KotloffN R Ruetsch
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D B RothM Gellert
Apr 1, 1993·The Journal of Experimental Medicine·S L TiegsD Nemazee
Apr 1, 1993·The Journal of Experimental Medicine·M Z RadicM Weigert
Apr 1, 1993·The Journal of Experimental Medicine·D GayM Weigert
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·D Melamed, D Nemazee
Oct 9, 1998·The Journal of Experimental Medicine·M W Retter, D Nemazee
Jul 3, 1999·Science·K HayakawaR R Hardy
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·V KouskoffD Nemazee
Aug 22, 2000·Analytical Chemistry·M SchuerenbergE Nordhoff

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Citations

Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pamela B NakajimaMelvin J Bosma
Dec 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shixue Shen, Tim Manser
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lenka YunkEline T Luning Prak
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laurent VerkoczyBarton F Haynes
Sep 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Miyo OtaDavid Nemazee
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patricia Y TsaoRobert A Eisenberg
Mar 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bao Hoa DuongDavid Nemazee
Dec 3, 2013·Immunologic Research·Wasif N KhanEmily S Clark
Jan 22, 2011·Annals of the New York Academy of Sciences·Eline T Luning PrakRobert A Eisenberg

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