Antigen Recognition Patterns of Intestinal Capillariasis Using Immunoblot-Based Serodiagnosis.

Acta Parasitologica
Marwa A HassanAbeer Said Abdel-Ghany Al-Antably

Abstract

Diagnosis of intestinal capillariasis depending on microscopic detection of parasitic stages is of low sensitivity, especially in cases with low worm burden. There is a necessity to develop sensitive and specific diagnostic tools of capillariasis for early treatment to avoid complications. Western blot (WB) technique showed promising results for antigen recognition patterns in several parasitic infections. This study is directed to identify and evaluate relevant proteins of intestinal capillariasis crude worm antigens using WB immunodiagnosis. Capillaria crude worm antigens were extracted and analyzed using SDS-PAGE. Sixty serum samples belonging to 3 groups (20 individuals each) were included; Group I (shedding Capillaria in feces), Group II (infected with other parasites) and Group III (negative parasitological results). Reactivity of the resulting bands of Capillaria with serum samples was analyzed using WB technique. Thirty-two immunoreactive bands were detected in WB analysis representing recognition of proteins with molecular weights (MW) varying from 19 to 110 kDa. Immunodominant proteins of 23.5, 31, 36.5, 40.5 and 44 kDa were recognized, respectively, in 35%, 30%, 85%, 95% and 75% of sera from patients with confirmed c...Continue Reading

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Methods Mentioned

BETA
ELISA
light microscopy
Electrophoresis

Software Mentioned

Gel pro - analyzer
statistics

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