PMID: 9071315Mar 1, 1997Paper

Antigen retrieval immunohistochemistry: past, present, and future

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
S R ShiC R Taylor

Abstract

The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting issues for the further development of AR. These issues include the use of a "test battery" and the concept of "maximal retrieval" applied to the selection of optimal test protocols for the standardization of AR.

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