PMID: 8599469Dec 1, 1995Paper

Antigen retrieval in immunofluorescent testing of bullous pemphigoid

The American Journal of Dermatopathology
K D'Ambra-CabryJ S Deng

Abstract

Direct immunofluorescence is an immunopathological technique frequently utilized for diagnosis of vesiculobullous disease such as bullous pemphigoid. Fresh-frozen tissue is required for immunofluorescent testing, making retrospective analysis difficult. In this study, we compared two methods of antigen retrieval in formalin-fixed, paraffin-embedded skin tissue from patients with bullous pemphigoid to determine if archival tissue, after use of an unmasking antigen, can be substituted for fresh-frozen tissue in the immunopathological study of skin. Paraffin-embedded tissue blocks from patients with bullous pemphigoid and patients with eosinophilic spongiotic dermatitis as the prodromal stage of bullous pemphigoid were obtained. Sections were mounted on poly-L-lysine-coated slides and the slides were deparaffinized. The methods of antigen retrieval included incubation with trypsin (0.1%) and microwave irradiation in urea (6 M). Antigen retrieval was followed by indirect immunofluorescence. Microwave irradiation was more effective in antigen retrieval than was incubation with trypsin (0.1%). Microwave irradiation in urea (6 M) produced more intense immunofluorescent staining than did trypsinization. Overall, positive basement membr...Continue Reading

Citations

May 17, 2011·Histochemistry and Cell Biology·Teruo HayashiTsung-Ping Su
Feb 1, 1997·The American Journal of Dermatopathology·I Al-RifaiA Claudy
Feb 23, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Shan-Rong ShiClive R Taylor
Nov 13, 1998·Diagnostic Cytopathology·D C Lapen, J Papillo

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