PMID: 6972044Mar 1, 1981Paper

Antigen-specific human T-cell clones: development of clones requiring HLA-DR-compatible presenting cells for stimulation in presence of antigen

Proceedings of the National Academy of Sciences of the United States of America
B SredniA S Fauci

Abstract

A method for cloning soluble antigen-specific proliferating human T lymphocytes directly from peripheral blood cells of individuals recently primed with the antigen is described. The soluble antigen was keyhole limpet hemocyanin. After expansion in liquid culture, these cells were shown to be antigen specific and to require HLA-DR-histocompatible presenting cells. Recloning of small numbers of these cells in soft agar under conditions of high plating efficiency yielded true clones (i.e., derived from a single cell) which retained specificity for the original stimulating antigen as shown by blastogenic responses measured by incorporation of [3H]thymidine. However, antigen-specific stimulation of clones was demonstrated only in the presence of an HLA-DR-compatible cooperating cell population together with the relevant antigen. Cells that manifested only HLA-A or B locus identity with the T-cell clone were not effective as presenting cells. Other antigens, such as tetanus toxoid, to which the donor of the clone or of the presenting cells was immune failed to stimulate the clone.

References

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Citations

Jan 1, 1982·Journal of Immunological Methods·B Sredni
Jan 1, 1984·Human Immunology·E Thorsby
Oct 28, 1986·Biochimica Et Biophysica Acta·M Zijlstra, C J Melief
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E C DeFreitasC M Croce
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S C MeuerE L Reinherz
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A BensussanM Sasportes
Nov 1, 1981·The Journal of Experimental Medicine·B SredniW E Paul
Aug 1, 1983·The Journal of Clinical Investigation·H C LaneA S Fauci
Dec 1, 1988·The Journal of Clinical Investigation·M A Liu, T Liu
Apr 1, 1982·Immunology Today·B Malissen, J Zeuthen
Jan 1, 1986·Microbiology and Immunology·M Kimoto, C G Fathman
Apr 15, 1983·Cellular Immunology·J BurnsR P Lisak
Mar 28, 2007·Seminars in Immunology·Sanmay BandyopadhyayFernando Macián
Dec 24, 1981·Nature·W E PaulR H Schwartz
Jan 1, 1982·Immunological Reviews·E ThorsbyH Nousiainen
Dec 1, 1982·Scandinavian Journal of Immunology·E J Berle, E Thorsby
Oct 1, 1983·Scandinavian Journal of Immunology·E QvigstadE Thorsby
Sep 1, 1985·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·E CelisT W Chang

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