Antigen-specific suppressor macrophages induced by culture with cyclosporine A plus acetylcholine receptor.

Journal of Neuroimmunology
K R McIntoshR W Kuncl

Abstract

Suppressor cells specific for the acetylcholine receptor (AChR) can readily be induced by culturing spleen cells from rats with experimental autoimmune myasthenia gravis in medium containing cyclosporine A plus AChR. We describe here the purification and characterization of novel antigen-specific suppressor cells, which appear to be macrophages, from these cultures. The cells were adherent to plastic, very large, and of low buoyant density. These properties were used to enrich the cells, first by adherence, followed by centrifugation on discontinuous Percoll density gradients. The enriched large suppressor cells (LSC) were shown to be potent suppressors of secondary immune responses to AChR, including both lymphoproliferation and antibody production. Furthermore, the LSC exhibited antigen specificity of suppression: LSC induced with AChR suppressed secondary antibody responses to AChR significantly more than to an unrelated antigen, keyhole limpet hemocyanin (KLH). In reciprocal experiments, LSC induced with KLH suppressed KLH responses significantly more than AChR responses. The LSC exhibited the morphologic, enzymatic, phenotypic, and radioresistance characteristics of macrophages. They were neither lymphocytes nor NK cells a...Continue Reading

References

Jan 1, 1978·The Journal of Experimental Medicine·C E Calkins, O Stutman
Jan 1, 1987·Annals of the New York Academy of Sciences·K R McIntosh, D B Drachman
Jul 1, 1985·The Journal of Experimental Medicine·R B SchwadronS Strober
Mar 24, 1988·The New England Journal of Medicine·R B Johnston
Jan 1, 1987·Annals of the New York Academy of Sciences·T R Johns
Nov 1, 1972·The Journal of Experimental Medicine·R EvansP Alexander
May 25, 1973·Science·J Patrick, J Lindstrom
Nov 1, 1973·Archives of Biochemistry and Biophysics·M E Eldefrawi, A T Eldefrawi
Nov 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·M B Rittenberg, K L Pratt
Jan 1, 1982·Immunological Reviews·G DoriaL Adorini
Jan 1, 1981·International Archives of Allergy and Applied Immunology·W PtakJ Marcinkiewicz
Nov 1, 1981·Cellular Immunology·F M Rollwagen, O Stutman
Mar 1, 1984·Annals of Neurology·R M PascuzziT R Johns

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Citations

Jun 21, 1993·Annals of the New York Academy of Sciences·D B DrachmanL Balcer
May 1, 1992·Journal of Neuroimmunology·K R McIntosh, D B Drachman
Jan 1, 1992·Current Eye Research·C D DijkstraJ G Damoiseaux
May 1, 1997·The Journal of Small Animal Practice·J A HallD C Twedt
Feb 13, 1996·Annals of the New York Academy of Sciences·D B DrachmanK R McIntosh

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