PMID: 6166714May 1, 1981Paper

Antigen-specific T lymphocyte clones. II. Purification and biological characterization of an antigen-specific suppressive protein synthesized by cloned T cells

The Journal of Experimental Medicine
M FresnoH Cantor

Abstract

We have generated an antigen-specific T suppressor clone that synthesizes 70,000-mol wt peptides that have antigen-specific-binding activity. Although these data also indicated that antigen-binding peptides completely inhibited the in vitro primary response to a complex antigen, suppression might reflect the combined biologic activities of many different 70-mol wt polypeptides or polypeptides associated with the 70,000-mol wt material by noncovalent interactions. The protein responsible for antigen-specific suppression was therefore purified to virtual homogeneity after sequential separation of internally labeled supernate peptides on Sephacryl S-200 and DEAE-cellulose columns followed by isoeleetrofocusing. The resulting protein is greater than 95 percent homogeneous according to sodium dodeeyl sulfate-polyacrylamide electrophoresis and represents two peptides having two very close but distinguishable isoelectric point values of approximately 5.0. The purified molecules are retained by columns coated with lentil lectin or antigen but not by columns coated with antisera specific for immunoglobulins, the I region of the major histocompatibility complex or Ly-1 or Ly-2 antigens. Less than 50 pg of the purified glycoprotein specif...Continue Reading

References

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Citations

Jan 1, 1982·Immunogenetics·M SuzanB Rubin
Jan 1, 1983·Molecular Aspects of Medicine·N R FaridJ C Bear
Apr 1, 1985·Molecular Immunology·R E Cone, K D Beaman
Oct 1, 1996·Cytokine & Growth Factor Reviews·G F Weber, H Cantor
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D R GreenD D Eardley
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R W RosensteinR K Gershon
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·K J WiederD R Webb
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W Ptak, R K Gershon
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R LifshitzE Mozes
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S MiyataniT Tada
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M M Abu-HadidG L Mayers
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PatarcaK A Johnson
Feb 1, 1982·The Journal of Experimental Medicine·A F Clark, J D Capra
Aug 1, 1982·The Journal of Experimental Medicine·M MinamiM E Dorf
Nov 1, 1985·The Journal of Experimental Medicine·S M HelfgottD E Trentham
Aug 2, 2003·Biology of Reproduction·Greg A JohnsonThomas E Spencer
Jan 1, 1982·Annals of the New York Academy of Sciences·B BenacerrafM E Dorf
Aug 1, 1984·Scandinavian Journal of Immunology·Z IkezawaJ Klein
Oct 1, 1982·Immunology Today·S Howie, W H McBride
Feb 1, 1982·Immunology Today·R H Schwartz
Oct 1, 1991·Cellular Immunology·M TakataA H Sehon
Sep 1, 1983·Immunology Today·J A Bluestone, R J Hodes
Apr 25, 2000·Biology of Reproduction·G A JohnsonF W Bazer
Aug 22, 2001·Biology of Reproduction·G A JohnsonR C Burghardt

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