Antigenic alteration of influenza B virus associated with loss of a glycosylation site due to host-cell adaptation

Journal of Medical Virology
Takehiko SaitoMasato Tashiro

Abstract

Effects of host-cell adaptation of the hemagglutinin (HA) protein were evaluated by the analyses of four pairs of recent influenza B field isolates, each pair consisting of an Madin Darby canine kidney (MDCK)- and an embryonated chicken egg-derived isolates from the same clinical specimen. Among the isolates examined, all of the MDCK-derived isolates retained glycosylation site at amino acid 197 on the HA1 molecule, whereas three egg-derived isolates lost it. Antigenic difference in the HA molecule between an MDCK- and an egg-derived isolates of three of these pairs was demonstrated to be associated with the glycosylation 197. Replication of the MDCK-derived isolates was suppressed in eggs, suggesting that the presence of the glycosylation 197 was disadvantageous to replication in eggs. Virus-binding affinity assay revealed that the loss of carbohydrate chain did not significantly alter the preferential recognition of sialic acid linkage. Immunogenicity and vaccine efficacy of an MDCK- and an egg-derived clones of B/Akita/27/2001, the former retained the glycosylation 197 and the latter lost it, were compared in a hamster model. When formalin-inactivated whole virion vaccines prepared from the paired isolates were administered ...Continue Reading

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Citations

Oct 9, 2008·Journal of Molecular Evolution·Baltazar NunesHelena Rebelo-de-Andrade
Aug 19, 2011·Journal of Virology·Elisabeth RoethlAndrej Egorov
Jan 11, 2008·Journal of Virology·Qinghua WangJianpeng Ma
Oct 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qinghua WangJianpeng Ma
Nov 26, 2008·Journal of Medical Virology·Jun ShenQinghua Wang
Jun 16, 2015·Influenza and Other Respiratory Viruses·Yipu LinJohn W McCauley
Oct 31, 2017·Nature Reviews. Microbiology·Velislava N Petrova, Colin A Russell
Jul 12, 2018·Influenza and Other Respiratory Viruses·Ching-Ping Tsai, Hsiang-Jung Tsai
Feb 27, 2020·The Journal of Infectious Diseases·Hector S IzurietaRichard Forshee
Dec 12, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia HütterBernd Lepenies
Mar 24, 2021·Expert Review of Vaccines·Pan Ge, Ted M Ross

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