Antigenic and Biological Characterization of ORF2-6 Variants at Early Times Following PRRSV Infection

Viruses
Alyssa B EvansS Carpenter

Abstract

Genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) challenges efforts to develop effective and broadly acting vaccines. Although genetic variation in PRRSV has been extensively documented, the effects of this variation on virus phenotype are less well understood. In the present study, PRRSV open reading frame (ORF)2-6 variants predominant during the first six weeks following experimental infection were characterized for antigenic and replication phenotype. There was limited genetic variation during these early times after infection; however, distinct ORF2-6 haplotypes that differed from the NVSL97-7895 inoculum were identified in each of the five pigs examined. Chimeric viruses containing all or part of predominant ORF2-6 haplotypes were constructed and tested in virus neutralization and in vitro replication assays. In two pigs, genetic variation in ORF2-6 resulted in increased resistance to neutralization by autologous sera. Mapping studies indicated that variation in either ORF2-4 or ORF5-6 could confer increased neutralization resistance, but there was no single amino acid substitution that was predictive of neutralization phenotype. Detailed analyses of the early steps in PRRSV replication in t...Continue Reading

References

Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J E CollinsH S Joo
Jul 1, 1991·The Veterinary Quarterly·G WensvoortF Wagenaar
Jan 10, 1995·Virology·J J MeulenbergG Wensvoort
Dec 13, 2002·Journal of Virology·Eric J SnijderWilly J M Spaan
Jan 7, 2003·Viral Immunology·Michael P MurtaughFederico Zuckermann
Oct 28, 2004·Veterinary Immunology and Immunopathology·O J Lopez, F A Osorio
May 12, 2007·Journal of Virology·Jay G CalvertSiao-Kun W Welch
Nov 15, 2008·The Journal of General Virology·Hanne Van GorpHans J Nauwynck
May 26, 2009·Vaccine·Tjeerd G KimmanNorbert Stockhofe-Zurwieden
May 27, 2009·Virology·M de LimaF A Osorio
Apr 23, 2010·The Journal of General Virology·Wander Van BreedamHans J Nauwynck
Jul 27, 2010·Virus Research·Siao-Kun W Welch, Jay G Calvert
Aug 10, 2010·Virus Research·Terje Dokland
Aug 31, 2010·Virus Research·Michael P MurtaughFrederick C-C Leung
Sep 15, 2010·Virus Research·Sarah CostersHans J Nauwynck
Apr 10, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Benjamin DelisleSylvie D'Allaire

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
AY545985

Methods Mentioned

BETA
glycosylation
in vitro transcription
transfection
light microscopy
PCR

Software Mentioned

ClustalW
SAS
MacVector
phyclust

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.