Antigenic and biological relationships between human coronavirus OC43 and neonatal calf diarrhoea coronavirus

The Journal of General Virology
G GernaM T Gerna

Abstract

Monospecific antisera were prepared in mice to human coronavirus OC43 and neonatal calf diarrhoea coronavirus (NCDCV) which had been previously adapted to growth in suckling mouse brain. Brain suspension from infected suckling mice was used as immunogen. The antigenic relationship between OC43 and NCDCV was studied by the indirect immunoperoxidase antibody technique, by the haemagglutination-inhibition (HI) test and a new infectious centre-reduction neutralization test. In mouse immune sera, a two-way cross-reaction between OC43 and NCDCV was detected. However, the antigenic relationship appeared to be closer for internal (as shown by immunoperoxidase staining) as compared to surface antigens (as shown by HI and neutralization). In primary infections of natural hosts there was a high degree of cross-reactivity between the two coronavirus strains for both surface and internal antigens, and homologous and heterologous titres were consistently within an eightfold dilution difference by all tests. Most human adults and calves had antibody to both OC43 and NCDCV and geometric mean titres of homologous antibody were higher than titres of heterologous antibody. Although OC43 and NCDCV share antigenic determinants, they possessed sever...Continue Reading

Citations

Jan 1, 1993·The British Veterinary Journal·M A Clark
Mar 12, 1999·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·J P LubyS Kurtz
Mar 25, 2014·Emerging Infectious Diseases·Benjamin MeyerChristian Drosten
Aug 13, 2013·The Lancet Infectious Diseases·Chantal B E M ReuskenMarion P G Koopmans
Apr 4, 2007·Expert Review of Anti-infective Therapy·Krzysztof PyrcLia van der Hoek
Oct 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·S Dea, S Garzon
May 29, 2020·Journal of Medical Virology·Nevio Cimolai
Jan 1, 1988·Journal of Tissue Culture Methods : Tissue Culture Association Manual of Cell, Tissue, and Organ Culture Procedures·Linda J SaifMohamed M Tarek
Dec 1, 1985·Journal of Clinical Microbiology·L J AndersonY O Stone

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.