Antigenic and physicochemical characterization of the 2nd International Standard for hepatitis B virus surface antigen (HBsAg)

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Christian G SchüttlerWolfram H Gerlich

Abstract

Standard preparations for HBsAg are required for quality control of test kits and clinical studies on HBsAg quantitation. WHO provides purified heat inactivated HBsAg diluted in negative defribinated plasma as 2nd International Standard (IS) for quality control of tests. Study of possible alterations of antigenicity, protein composition, size and density of the heat inactivated source material (SM) for the 2nd IS. Native HBsAg and SM were examined by quantitative immune electrophoresis (QIE), SDS-PAGE, ultracentrifugation and gel chromatography. HBV DNA was sequenced and the HBsAg geno/subtype derived. The SM contained 97,600 International Units HBsAg/ml in QIE which agreed very well with the previous evaluations by WHO using 10 different assays. In SDS-PAGE, SM showed on a strong background the small HBs proteins but no preS proteins. SM had a more heterogeneous density than native HBsAg and contained particle aggregates. The HBsAg geno/subtype of SM was A2/adw2. The IS has very good HBs antigenicity, but it lacks the preS domains, has modified HBs proteins and is partially aggregated. While it has been proven very useful for quality control of tests, certain inconsistencies due to the altered structure of its HBsAg cannot be ...Continue Reading

References

Apr 28, 2006·The New England Journal of Medicine·Oliver SchildgenWolfram H Gerlich
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karsten WursthornJoerg Petersen
Aug 22, 2008·Journal of Hepatology·Giovanni RaimondoFabien Zoulim
Dec 31, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rami MoucariPatrick Marcellin
Apr 2, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Maurizia Rossana BrunettoPatrick Marcellin

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Citations

Jan 17, 2015·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Akihiro MatsumotoEiji Tanaka
Nov 23, 2011·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Akihiro MatsumotoHiromitsu Kumada
Jul 9, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M ChudyW H Gerlich
Dec 20, 2014·Medical Microbiology and Immunology·Wolfram H Gerlich
Jun 28, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Pia L SeizDieter Glebe
Jun 28, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Dianna E WilkinsonUNKNOWN Collaborative Study Group

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