Antigenic Determinants of Possible Vaccine Escape by Porcine Circovirus Subtype 2b Viruses

Bioinformatics and Biology Insights
Megan ConstansSheela Ramamoorthy

Abstract

Currently available commercial vaccines against porcine circovirus strain 2 (PCV2) solely target the PCV2a genotype. While PCV2 vaccines are highly effective in preventing clinical signs, PCV2b has dominated over the PCV2a genotype in prevalence, corresponding with the introduction of PCV2a vaccines. A recently emerged PCV2b recombinant with an additional amino acid in the capsid protein, designated the mutant PCV2b (mPCV2b), is cause for concern due to its increased virulence and rapid spread. The accumulation of recent evidence for the increased genetic diversity in PCV2 suggests that current vaccines against PCV2a may be inducing selection pressure and driving viral evolution. In this study, the hypothesis that differences in key immune epitopes between the PCV2a vaccine strains, a classical PCV2b strain called PCV2b 41513 obtained from a vaccine-failure case, and mPCV2b strains could promote vaccine escape was tested using immuno-informatic tools. In the major viral proteins, 9 of the 18 predicted swine leukocyte antigens (SLA) class-I epitopes, 8 of the 22 predicted SLA class-II epitopes, and 7 of the 25 predicted B cell epitopes varied between the vaccine and field strains. A majority of the substitutions in both the T- a...Continue Reading

References

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Citations

Oct 23, 2016·Veterinary Microbiology·Zahra AfghahSheela Ramamoorthy
May 27, 2016·Journal of Clinical Microbiology·Yunfeng SongSheela Ramamoorthy
Nov 13, 2017·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Danielle GavaRejane Schaefer
Jul 3, 2019·Veterinary Sciences·Oleksandr KolyvushkoSheela Ramamoorthy

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Datasets Mentioned

BETA
KR816332
JX535297

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

Propred
LaserGene core suite
Clustal
Jalview
MegAlign Pro
MegAlign
Lasergene
Pymol
NetMHCpan

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