Antigenic differences within the Cryptosporidium hominis and Cryptosporidium parvum surface proteins P23 and GP900 defined by monoclonal antibody reactivity

Molecular and Biochemical Parasitology
Gregory D SturbaumMichael W Riggs

Abstract

The biological basis for the specificity of host infectivity patterns of Cryptosporidium spp., in particular C. hominis and C. parvum, has yet to be fully elucidated. Comparison of the C. parvum and C. hominis P23 and GP900 predicted amino acid sequences revealed 3 differences in P23 and 4 and 17 differences in GP900 domains 1 and 5, respectively. Using monoclonal antibodies developed against the surface (glyco)proteins P23 and GP900 of the C. parvum Iowa isolate, solubilized glycoprotein from three C. hominis isolates was screened for reactivity using Western immunoblots. One of ten P23 MAbs and three of 21 GP900 MAbs were not reactive with any of the three C. hominis isolates. The non-reactive P23 MAb binds to a peptide epitope, while the non-reactive GP900 MAbs bind to either carbohydrate/carbohydrate-dependent or peptide epitopes of C. parvum. These results demonstrate phenotypic differences between C. hominis and C. parvum within two (glyco)proteins that are involved in parasite gliding motility and attachment/invasion.

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Citations

Jan 27, 2012·The Journal of Infectious Diseases·Abhineet SheoranSaul Tzipori
Sep 8, 2011·The American Journal of Tropical Medicine and Hygiene·Olivia LaiRoberta O'Connor
Feb 4, 2012·The American Journal of Tropical Medicine and Hygiene·Anoli J BoradHonorine D Ward
Mar 8, 2014·The Journal of Parasitology·Mark C JenkinsRonald Fayer
Feb 2, 2010·The Veterinary Clinics of North America. Food Animal Practice·Carol R WyattRonald Fayer
Oct 28, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Silvia Cristina OsakiVanete Thomaz Soccol
Apr 1, 2014·Parasitology·Matthias Lendner, Arwid Daugschies

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