Antigenic evidence of prevalence and diversity of Mycobacterium tuberculosis arabinomannan

Journal of Clinical Microbiology
Aharona Glatman-FreedmanMichael Shapiro

Abstract

Arabinomannan (AM) is a polysaccharide of the mycobacterial capsule. The capsular polysaccharides of various microorganisms are diverse, and this diversity is important for classification of organisms into serotypes and vaccine development. In the present study we examined the prevalence and diversity of AM among Mycobacterium tuberculosis strains using four AM-binding monoclonal antibodies (MAbs). One of these MAbs, MAb 9d8, is known to bind to AM specifically. By whole-cell enzyme-linked immunosorbent assay (ELISA), the AM recognized by MAb 9d8 was detected on the surfaces of 9 of 11 strains, while 2 strains showed no reactivity with MAb 9d8. However, the AM recognized by MAb 9d8 was found in the culture supernatants of all 11 M. tuberculosis strains tested, as demonstrated by capture ELISA. Other AM-binding MAbs reacted both with the surfaces and with the culture supernatants of all 11 strains. Mice immunized with an experimental AM-recombinant Pseudomonas aeruginosa exoprotein A (rEPA) conjugate vaccine had an increased antibody response to AM and a moderate reduction in the numbers of CFU in their organs 7 days after challenge. Our results indicate that AM was detected in all M. tuberculosis strains tested, with difference...Continue Reading

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Citations

Nov 5, 2010·Clinical and Vaccine Immunology : CVI·Michael SievJacqueline M Achkar
Jan 24, 2006·Infection and Immunity·Mark L Lang, Aharona Glatman-Freedman
Sep 9, 2008·Trends in Microbiology·Gunilla KälleniusStefan B Svenson
Jul 17, 2009·Clinical and Experimental Immunology·F Abebe, G Bjune
Feb 24, 2015·Immunological Reviews·Jacqueline M AchkarArturo Casadevall
Mar 19, 2013·Cell Host & Microbe·Jacqueline M Achkar, Arturo Casadevall
Jan 15, 2020·The Journal of Clinical Investigation·Tingting ChenJacqueline M Achkar
Apr 21, 2007·Journal of Medical Microbiology·Ayelet BarenholzHervé Bercovier
Dec 24, 2010·Clinical and Vaccine Immunology : CVI·Nadine G RouphaelSandra Romero-Steiner
Jan 11, 2020·Frontiers in Immunology·Milla R McLeanAmy W Chung
Jul 20, 2019·The Biochemical Journal·Rainer KalscheuerRafael Prados-Rosales
Oct 2, 2019·Frontiers in Immunology·Margarida Correia-NevesGunilla Källenius
Mar 9, 2021·Frontiers in Immunology·Willemijn F RijninkSimone A Joosten
Mar 9, 2011·The Journal of Organic Chemistry·Changalvala V S RamamurtyBert Fraser-Reid

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