Antigenic importance of the carboxy-terminal beta-strand of the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Clinical and Diagnostic Laboratory Immunology
Sarah K WoottonDongwan Yoo

Abstract

Five domains of antigenic importance were previously mapped on the nucleocapsid protein (N) of the porcine reproductive and respiratory syndrome virus (PRRSV), and a domain comprised of the 11 C-terminal-most amino acids (residues 112 to 123) was shown to be essential for binding of N-specific conformation-dependent monoclonal antibodies (MAbs). In the present study, the importance of individual residues within this C-terminal domain for antigenicity was investigated using eight different mutant constructs of N expressed in HeLa cells. Single amino acid substitutions were introduced into the C-terminal domain of the N protein, and the significance of individual amino acids for MAb reactivity was determined by immunoprecipitation. None of the MAbs tested recognized the mutant with a leucine-to-proline substitution at residue 114 (L114P), while V112P, R113P, R113D, I115P, and R116P reduced MAb binding significantly. Conversely, substitution of amino acids at positions 118 (T118S) and 121 (P121A) had little effect on MAb binding. Secondary-structure predictions indicate that amino acids 111 to 117 form a beta-strand. In view of the fact that replacement of beta-strand-forming amino acids with proline elicited the greatest effect o...Continue Reading

References

Sep 15, 1977·Journal of Molecular Biology·P Y Chou, G D Fasman
May 1, 1986·Virology·J L SagripantiR Weinmann
Jul 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·K J YoonK B Platt
Apr 17, 1998·Veterinary Pathology·K D Rossow
Nov 5, 1998·Clinical and Diagnostic Laboratory Immunology·S K WoottonD Yoo
Feb 3, 1999·Bioinformatics·J A CuffG J Barton
Jul 10, 1999·Journal of Virology·E J SnijderA A de Vries
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·G van MarleE J Snijder

❮ Previous
Next ❯

Citations

Jun 12, 2009·Archives of Virology·Hakimeh MohammadiDongwan Yoo
Mar 27, 2013·Veterinary Microbiology·Tomasz StadejekMartin B Oleksiewicz
May 5, 2007·Vaccine·Kelly-Anne SpencerJulian A Hiscox
Oct 28, 2004·Veterinary Immunology and Immunopathology·Dongwan YooJay G Calvert
Dec 3, 2014·Veterinary Microbiology·Mingyuan Han, Dongwan Yoo
Feb 7, 2012·Biochemical and Biophysical Research Communications·Stefanie S JourdanJulian A Hiscox
Dec 12, 2012·Veterinary Microbiology·Jean-Pierre FrossardTrevor W Drew
Jun 18, 2019·Journal of Theoretical Biology·Janani Srree Murallidharan, Stephen J Payne

❮ Previous
Next ❯