Abstract
Amoebiasis, caused by Entamoeba histolytica, is one of the leading parasitic infections in the world. This study was aimed at profiling antigenic membrane proteins of a virulent variant of E. histolytica HM-1:IMSS. The membrane proteins were extracted using ProteoExtract® kit (Merck, Darmstadt, Germany) or conventional method, separated using OFFGEL 3100 fractionator (Agilent Technologies, Santa Clara, California), followed by SDS-PAGE and Western blot analysis. Selected antigenic membrane proteins were identified using LC-ESI-MS/MS. Subsequently, the proteins were classified according to their biological processes and predictions were made on membrane and membrane-associated proteins. When the proteins were probed with pooled sera from amoebic liver abscess (ALA) patients, 10 and 15 antigenic proteins with molecular weights 25 to 200 kDa were identified using the ProteoExtract® kit and conventional method, respectively. LC-ESI-MS/MS identified 13 antigenic proteins, and both extraction methods predicted six of them as membrane and membrane-associated proteins. The topmost biological processes which comprised of six proteins were involved in cellular processes.. These antigenic membrane proteins merit further investigations as ...Continue Reading
References
Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Aug 1, 1994·Trends in Genetics : TIG·S Fields, R Sternglanz
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A Fancy, T Kodadek
Aug 31, 2002·The American Journal of Tropical Medicine and Hygiene·Joerg BlessmannEgbert Tannich
Sep 13, 2002·The American Journal of Tropical Medicine and Hygiene·Omar M Amin
Mar 28, 2003·Lancet·Samuel L Stanley
Sep 4, 2003·Genome Research·Paul D ThomasApurva Narechania
Jan 24, 2006·Infection and Immunity·Rashidul HaqueWilliam A Petri
Nov 23, 2006·Antioxidants & Redox Signaling·Christopher Horst Lillig, Arne Holmgren
Mar 17, 2007·Experimental and Molecular Pathology·A Olivos-GarcíaR Pérez-Tamayo
Dec 19, 2007·Infection and Immunity·Jose E Teixeira, Christopher D Huston
Dec 17, 2008·International Journal for Parasitology·Espiridión Ramos-MartínezRuy Pérez-Tamayo
Feb 6, 2010·Foodborne Pathogens and Disease·Nurulhasanah OthmanRahmah Noordin
May 18, 2010·Journal of Proteomics & Bioinformatics·Leepika Tuli, Habtom W Ressom
May 27, 2010·Electrophoresis·Hidetaro YasumitsuHiroshi Katsuno
Nov 30, 2012·Nucleic Acids Research·Huaiyu MiPaul D Thomas
Dec 19, 2012·Lancet·Rafael LozanoZiad A Memish
Jan 2, 2014·BMC Infectious Diseases·Yi-Tsung LinChang-Phone Fung
Feb 8, 2014·Human Vaccines & Immunotherapeutics·Jeanie QuachKris Chadee
Feb 7, 2015·Biotechnology Advances·E GünerkenR H Wijffels
Oct 21, 2015·Frontiers in Microbiology·Rosa M Andrade, Sharon L Reed
Jul 5, 2017·PeerJ·Kumar Manochitra, Subhash Chandra Parija