Antigenic modulation and turnover in human neutrophils

The Journal of Clinical Investigation
S A WeitzmanT P Stossel

Abstract

Turnover of membrane constituents appears important in many biologic processes. We studied this process in neutrophils by immunologic methods. The capacity of neutrophils to recognize other neutrophils coated with antibodies against membrane antigens was used to determine the changes that occur after attachment of the antibody to the neutrophil membrane. Neutrophils were sensitized for 30 min at 22 degrees C with antibodies from three patients with antineutrophil autoantibodies. The sensitized neutrophils were recognized by normal neutrophils, which responded with an increase in glucose oxidation. If, after sensitization, the sensitized neutrophils were not immediately exposed to normal neutrophils, but instead were incubated at 37 degrees C for varying times, the capacity to elicit a recognition response decayed and was gone by 30 min. Additionally, the capacity of the cells to be resensitized by reexposure to antibody also decayed during this period. However, after further incubation at 37 degrees C, the neutrophils recovered the capacity to become sensitized; and this recovery was not inhibited by the addition of cycloheximide. Control incubations with normal immunoglobulin (Ig)G did not elicit a recognition response. The de...Continue Reading

References

Aug 1, 1976·The Journal of Cell Biology·C R Kahn
May 20, 1978·Lancet·S A Weitzman, T P Stossel
Oct 9, 1975·The New England Journal of Medicine·L A BoxerT P Stossel

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Citations

Jan 1, 1981·Veterinary Clinical Pathology·W R Chickering, K W Prasse
Dec 1, 1982·The Journal of Clinical Investigation·P K RustagiG L Logue
Jan 1, 1985·The Journal of Clinical Investigation·S A WeitzmanE C Ridgway
Aug 1, 1994·The American Journal of the Medical Sciences·K R Hartman
Jan 1, 1984·Immunology Today·L Chatenoud, J F Bach

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