PMID: 6981815Aug 1, 1982Paper

Antigenic polypeptide fragments of a receptor related to the Fab fragment of human immunoglobulin from thymus-derived lymphocytes

Proceedings of the National Academy of Sciences of the United States of America
J J MarchalonisA C Wang

Abstract

Certain thymus-derived lymphocytes of man and other primates express surface components related to the variable region of immunoglobulin heavy chains but lack constant region determinants defining any known immunoglobulin class. To obtain structural information on this molecule that can be used for comparison with known immunoglobulins and other surface molecules, we isolated the T cell-derived molecule by affinity chromatography using an antiserum raised against the monomeric Fab fragment of a human Waldenström macroglobulin and subjected the isolated molecule to either proteolysis using enzymes or cleavage with cyanogen bromide, followed by isolation of polypeptides which bore the Fab- or heavy chain variable region (VH)-related antigenic markers. The intact T cell molecule had an apparent mass of 68,000 daltons and no evidence was found for covalent or noncovalent association with polypeptides resembling light chains in apparent mass. The pattern of fragments obtained by cleavage of the T cell heavy chain suggests that the molecule is comprised of domains of approximate mass 12,000 daltons. Isolation of fragments from the digests that bear Fab-related serological markers shows that the molecule can be degraded into fragments...Continue Reading

References

Jan 1, 1978·Journal of Cellular Physiology·K IshiiJ I Furuyama
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jun 1, 1981·Journal of Immunogenetics·J J Marchalonis, A C Wang
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J J MarchalonisF Karush
Dec 1, 1981·Journal of Immunogenetics·J J Marchalonis, A J Strelkauskas
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R E ConeR K Gershon

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