PMID: 25784097Mar 19, 2015Paper

Antigenic profile of muscularis mucosae and muscularis propria of the urinary bladder

Iranian Journal of Immunology : IJI
Sławomir PoletajewBarbara Górnicka

Abstract

Differentiation between the muscularis mucosae (MM) and muscularis propria (MP) of the bladder remains challenging. To identify MM- and MP-specific antigens that could be of potential value for staging of urothelial carcinoma in a pilot study. The expression of 12 protein antigens in 11 human bladder specimens was examined. There were 5 post radical cystectomy specimens and 6 normal bladder autopsy specimens. Antibodies against actin, caldesmon, type IV collagen, cytokeratin, desmin, elastin, fibronectin, filamin, laminin, miotilin, smoothelin, and vimentin were used. Slides were stained with immunohistochemical reagents and assessed using light microscopy. The intensity of the immune reaction within MM and MP was evaluated in a four-level scale as negative and weakly, moderately or strongly positive. The presence of MM was noticed in 63.6% of specimens. The expression of desmin, filamin, and smoothelin was stronger within MP compared to MM in all cases. Stronger reaction with anti-type IV collagen antibodies was noticed within MP in 80% of the cases. In the whole study group, the expression of vimentin was stronger within MM than MP. MM and MP cells are of different antigenic characteristics. This can be used in the microscopi...Continue Reading

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