Antigenic properties and diagnostic potential of African swine fever virus protein pp62 expressed in insect cells

Journal of Clinical Microbiology
Carmina GallardoJose Manuel Sánchez-Vizcaíno

Abstract

African swine fever (ASF) is an infectious and economically important disease of domestic pigs. The absence of vaccine renders the diagnostic test the only tool that can be used for the control of new outbreaks of the disease. At present, the enzyme-linked immunosorbent assay (ELISA) test is the most useful method for large-scale ASF serological studies, although false positives have been detected, mainly on poorly preserved sera. In order to improve the current diagnostic test available for ASF, we have studied the antigenic properties of the ASF virus polyprotein pp62 and its suitability for use in a novel ELISA. Two well-known antigenic proteins of ASF virus, p32 and p54, were also included in this study. These proteins were expressed in the baculovirus expression system and used as antigens in ASF serological tests. Our results indicate that the use of these recombinant proteins as antigens in the ELISAs improves the sensitivity and specificity obtained with the conventional diagnosis test used to detect antibodies against ASF virus. Furthermore, the use of polyprotein pp62 in an ELISA for testing poorly preserved sera allows performance of the diagnosis of ASF without the need to confirm the results by the immunoblot test....Continue Reading

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Citations

Jan 18, 2012·Transboundary and Emerging Diseases·L MurJ M Sánchez-Vizcaíno
Jul 9, 2016·Transboundary and Emerging Diseases·A S KazakovaA D Sereda
Oct 9, 2012·Virus Research·Patricia de LeónAngel L Carrascosa
Jan 8, 2013·Virus Research·Christopher L Netherton, Thomas E Wileman
Sep 25, 2017·Transboundary and Emerging Diseases·M AriasJ M Sánchez-Vizcaíno
Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
May 8, 2009·Clinical and Vaccine Immunology : CVI·Carmina GallardoAlexandre Leitão
Jul 7, 2018·Journal of Veterinary Research·Ning JiaJie Zhang
Jun 2, 2020·Frontiers in Veterinary Science·Natasha N GaudreaultJuergen A Richt
Dec 23, 2015·Porcine Health Management·Ma Carmen GallardoMa Luisa Arias
Jul 7, 2020·Porcine Health Management·Laia Bosch-CamósFernando Rodriguez
Aug 18, 2017·Journal of Virology·Paula L MonteagudoFernando Rodríguez
Feb 20, 2021·Bioorganic & Medicinal Chemistry·Bangzuo LiuZeliang Chen
Nov 8, 2021·Transboundary and Emerging Diseases·Tridib Kumar RajkhowaSheityabati Sagolsem

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