PMID: 3765372Jan 1, 1986Paper

Antigenic properties of NDV glycoproteins synthesized in the presence of 2-deoxy-d-glucose

Veterinarno-meditsinski nauki
L Vasileva, I Neĭcheva

Abstract

Experiments were carried out to establish whether the glucose antimetabolite, 2-desoxi-d-glucose (DDG), effected the proteolytic breakdown of the envelope proteins of NDV and if there was any effect on there antigenic properties. It was found that it inhibited the glucose action on the NDV envelope proteins, the hemagglutinin-neuraminidase (HN), and the F-protein so that their biologic properties were not manifested, and the production of infectious virus was blocked. In the presence of DDG the virus-infected were shown to contain the virus envelope proteins, the membrane protein, and the nucleocapsid both through electrophoresis in 10 percent polyacrylamide gel and through the agar gel immunodiffusion test (with the use of specific rabbit anti-HN, anti-F protein, and anti-M protein sera). The antigenic properties of the two glycoproteins of the NDV envelope did not change under the action of the DDG inhibitor as seen by the precipitation lines of the control variants (cells infected with the virus but not treated with the antimetabolite), which were identical with those obtained with the test variants (cells treated with DDG).

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.