Antigenic relevance of F protein in chronic hepatitis C virus infection

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Florence Komurian-PradelGlaucia Paranhos-Baccalà

Abstract

The hepatitis C virus (HCV) F protein is a recently described, frameshift product of HCV core encoding sequence of genotype 1a. Its function and antigenic properties are unknown. Using enzyme-linked immunosorbent assay, we assessed the prevalence of anti-F antibodies in 154 patients chronically infected with HCV, 65 patients with other liver diseases, and 121 healthy controls. For this purpose, we expressed a highly purified HCV F recombinant protein from HCV genotype 1a in Escherichia coli. Because the F protein shares the 10 first amino acids with the core protein, the anti-HCV F response was also assessed by a F recombinant protein deleted of its 10 first amino acids [Delta(1-10)-F]. Ninety-six (62%) of the 154 HCV serum samples reacted with the complete F recombinant protein, whereas 39 (25%) showed a weaker anti-Delta(1-10)F reactivity and 150 (97%) had anti-core antibodies. No reactivity against F, Delta(1-10)F, or core was detected in any of the controls. To exclude a potential cross-reaction of anti-F antibodies with anti-core antibodies, a specific enzyme-linked immunosorbent assay was performed for anti-core antibodies. The specificity of anti-F antibodies was confirmed using an F synthetic peptide. The prevalence of ...Continue Reading

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Citations

Oct 31, 2008·Journal of Virology·Kamile YuksekJing-Hsiung James Ou
Feb 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Laura K McMullanCharles M Rice
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May 1, 2013·Biochemical and Biophysical Research Communications·Wen-Ta HuShih-Yen Lo
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Apr 12, 2015·Pathogens and Disease·Tayebeh HashempourShahin Merat
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Apr 26, 2019·Genes and Immunity·Stanislas Pol, Sylvie Lagaye
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