Antigenic specificity of porcine T cell response against foot-and-mouth disease virus structural proteins: identification of T helper epitopes in VP1

Virology
A RodríguezF Sobrino

Abstract

The contribution of each of the viral capsid proteins of foot-and-mouth disease virus (FMDV) in the T cell response of vaccinated pigs has been studied. Viral polypeptides, VP1 to VP4, were expressed as fusion proteins in Escherichia coli, and were used to stimulate peripheral blood mononuclear cells of vaccinated animals. Significant, dose-dependent responses to whole virion were detected in the seven animals analyzed and, in five of them, responses to recombinant polypeptides VP1, VP2, and VP3 were noticed, VP4 was recognized only by one of the pigs. Among the responder animals, VP1 and VP3 induced the higher proliferative responses. The patterns of recognition of a nested set of VP3 fragments expressed as fusions in E. coli were different among the animals studied and were consistent with the presence of different T cell epitopes on the protein. Likewise, three of the four VP1 fragments induced significant responses and were differentially recognized by each of the animals tested. Partially overlapping synthetic peptides spanning VP1 amino acids 41 to 209 were used to identify T cell epitopes in this protein. The significant responses obtained in three of seven additional FMDV vaccinated outbred pigs analyzed revealed the ex...Continue Reading

Citations

Sep 6, 2011·Virology Journal·Li-na MaYong-sheng Liu
Feb 14, 2002·The Journal of General Virology·Elisenda ArmengolArmin Saalmüller
Jul 17, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Tatsuhiko AzegamiYoshikazu Yuki
Feb 20, 2002·Biologicals : Journal of the International Association of Biological Standardization·C Y WangF Brown
Jan 9, 2020·AMB Express·Mpho Victoria MamaboloMichael Crampton

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