PMID: 6163993Apr 23, 1981Paper

Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies

Nature
W GerhardR Webster

Abstract

The recurrence of influenza virus infection in man is attributed primarily to changes occurring in the antigenic structure of the viral surface glycoproteins, especially of the haemagglutinin (HA) molecule. Comparative antigenic analysis of epidemic influenza virus strains has allowed the description of 'strain-specific' and 'cross-reactive' antigenic determinants. However, the interpretation of these findings remained ambiguous, because the specificity of the applied antisera was insufficiently defined and because the antigenic differences among the HA molecules of various epidemic virus strains resulted presumably from a large number of amino acid substitutions. Thus, in characterizing the antigenic structure of the HA molecule, our approach has been (1) to generate a panel of monoclonal anti-HA hybridoma antibodies, (2) to use some of these antibodies to select mutants of the influenza A/PR/8/34 (PR8) virus expressing antigenically altered HA molecules, and (3) to construct an operational antigenic map of the HA molecule by comparative antigenic analysis of the mutant viruses with the monoclonal antibodies. As we report here, analysis of the 34 mutant viruses selected has enabled us to define four antigenic sites on the HA m...Continue Reading

References

Jan 1, 1975·Advances in Immunology·M Reichlin
Jan 1, 1979·British Medical Bulletin·C Stuart-Harris
Aug 2, 1972·Nature: New Biology·C M Brand, J J Skehel
Dec 1, 1970·The Journal of General Virology·G C Schild

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Citations

Feb 1, 1985·European Journal of Clinical Microbiology·H Krakauer
Nov 8, 2008·Archives of Virology·Masatoshi OkamatsuHiroshi Kida
Mar 25, 2009·Immunology and Cell Biology·Lorena E Brown, Anne Kelso
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Suman R DasJonathan W Yewdell
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yan HanShu-Bing Qian
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E D KilbourneC W Whitaker
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R M Iorio, M A Bratt
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M A JabbarD P Nayak
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M L Dyall-SmithI H Holmes
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S L Zebedee, R A Lamb
Oct 1, 1986·The Journal of Cell Biology·C S CopelandA Helenius
Aug 28, 2001·AIDS Research and Human Retroviruses·S D'CostaJ L Hurwitz

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