Antigenicity and vaccine potential of Marburg virus glycoprotein expressed by baculovirus recombinants

Virology
M HeveyA Schmaljohn

Abstract

There is no effective vaccine for Marburg virus (MBGV) or any other filovirus, nor enough pertinent information to expedite rational vaccine development. To ascertain some of the minimal requirements for a MBGV vaccine, we determined whether whole inactivated MBGV, or a baculovirus-expressed virion subunit, could be used to immunize guinea pigs against a lethal infection. Baculovirus recombinants were made to express the MBGV glycoprotein (GP) either as a full-length, cell-associated molecule or a slightly truncated (5.4%) product secreted into medium; the latter, for its far greater ease in manipulation, was tested for its vaccine potential. Like MBGV GP, both the full-length and truncated GP expressed by baculovirus recombinants were abundantly glycosylated with both N- and O-linked glycans; differences in glycosylation were detectable, but these could not be shown to affect antigenicity with respect to available antibodies. The recombinant truncated glycoprotein elicited protection against lethal challenge with the MBGV isolate from which it was constructed and less effectively against an antigenically disparate MBGV isolate. Killed (irradiated) MBGV antigen was protective, in a reciprocal fashion, against both MBGV types. I...Continue Reading

References

Mar 1, 1987·Virology·C S SchmaljohnJ M Dalrymple
Nov 1, 1985·Virology·L H ElliottJ B McCormick
Nov 25, 1967·Lancet·C E SmithI Zlotnik
Apr 10, 1982·Lancet·D H SmithW K Keruga
Dec 1, 1983·The Journal of Infectious Diseases·C S SchmaljohnJ M Dalrymple
Apr 1, 1981·Journal of Clinical Microbiology·J B MoeH W Lupton
May 1, 1994·Biological Chemistry Hoppe-Seyler·E KretzchmarH D Klenk
Jan 1, 1993·Archives of Virology. Supplementum·H FeldmannA Sanchez
Oct 1, 1995·In Vitro Cellular & Developmental Biology. Animal·T R Davis, H A Wood
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SanchezS T Nichol
Dec 1, 1995·Virus Research·T W Geisbert, P B Jahrling

❮ Previous
Next ❯

Citations

Apr 2, 2005·Nature Reviews. Drug Discovery·James C BurnettSina Bavari
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Waranyoo PhoolcharoenMelissa M Herbst-Kralovetz
Mar 6, 2002·The Journal of Experimental Medicine·Sina BavariM Javad Aman
Jul 14, 2004·The Journal of Experimental Medicine·Kelly L WarfieldSina Bavari
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Dec 6, 2007·The Journal of Infectious Diseases·Loreen L LoftsAlan L Schmaljohn
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Jan 5, 2007·Viral Immunology·Mansour MohamadzadehAlan L Schmaljohn
Oct 19, 2011·The Journal of Infectious Diseases·Kelly L Warfield, M Javad Aman
Jun 21, 2013·Science Translational Medicine·Lisa M JohansenGene G Olinger
Oct 27, 2007·Virology Journal·Kelly L WarfieldSina Bavari
Oct 14, 2011·Viruses·Steven B Bradfute, Sina Bavari
Nov 22, 2012·Viruses·Steven B BradfuteMike Bray
Dec 4, 2012·Viruses·Kristina BrauburgerJudith Olejnik
Nov 3, 2011·Future Virology·Masfique MehediHideki Ebihara
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kelly L WarfieldSina Bavari
Jan 19, 2012·Journal of Biomedicine & Biotechnology·Kelly Lyn Warfield, Gene Garrard Olinger
Oct 19, 2011·The Journal of Infectious Diseases·JoLynne RaymondMike Bray
Oct 24, 2001·Vaccine·M HeveyA L Schmaljohn
Jun 26, 2007·Nature Reviews. Immunology·Mansour MohamadzadehAlan L Schmaljohn
Apr 30, 2009·Expert Review of Anti-infective Therapy·Keith E SteeleMansour Mohamadzadeh
Jul 20, 2005·Expert Review of Vaccines·Kelly L WarfieldSina Bavari
Feb 7, 2007·Expert Review of Vaccines·Daniel G Bausch, Thomas W Geisbert
Apr 9, 2008·Expert Review of Vaccines·Chinglai YangRichard W Compans
Oct 20, 2009·Vaccine·Shan Lu, Shixia Wang
Dec 8, 2007·Molecular Aspects of Medicine·Paolo AscenziGiuseppe Ippolito
Mar 27, 2007·Cellular Microbiology·Claudette L FullerSina Bavari
Jan 24, 2007·Vaccine·Douglas S Reed, Mansour Mohamadzadeh
May 3, 2006·Lancet·Stephan Becker
May 17, 2005·Journal of Virological Methods·George KallstromM Javad Aman
Oct 2, 2015·Viruses·Scott M Anthony, Steven B Bradfute
Dec 12, 2001·Molecular and Cellular Probes·T R GibbE A Henchal
Mar 24, 2016·Pathogens and Disease·Alan Schmaljohn, George K Lewis
Feb 23, 2000·Virology·V E VolchkovH Feldmann

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.