Antigenicity of adult Schistosoma mansoni alkaline phosphatase

Parasite Immunology
F H Pujol, I M Cesari

Abstract

Antibodies to the alkaline phosphatase (AP) of Schistosoma mansoni in infected human and mice sera were evaluated by a direct solid-phase AP immunoadsorption assay (APIA) and by Western blot and immunostaining. APIA consisted of (a) solid-phase capture of immunoglobulins from infected human or mice, (b) immunoadsorption of the enzyme antigen by the antibodies, and (c) detection of the enzymatic activity. By this procedure the appearance of the anti-AP response in mice was detected around 50 days post-infection; this response was not specific for an AP of a given schistosome strain and it was not induced by an autoimmunity phenomenon. Fourteen out of 15 sera from infected people tested by APIA showed a clear antibody response against this enzyme. Immunoblots in non-reducing conditions supported APIA results indicating that the parasite AP was specifically recognized by the antibodies present in infected human and mice sera. These results suggest the possible usefulness of the schistosome AP as a marker for S. mansoni infection.

References

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Citations

Jan 19, 2006·Parasitology Research·Nelmar GarcíaRenzo Nino Incani
Apr 1, 1990·Molecular and Biochemical Parasitology·F H PujolI M Cesari
Oct 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P LawtonA F Petavy
Aug 6, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·I M CesariD Richard-Lenoble
Jul 3, 2013·PLoS Neglected Tropical Diseases·Guidenn SulbaránItalo M Cesari
May 12, 2009·Molecular and Biochemical Parasitology·Henrique K RofattoLeonardo P Farias
Sep 18, 1999·The Veterinary Journal·I Fairweather, J C Boray
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Belkisyolé Alarcón de NoyaOscar Noya
Nov 12, 2002·Memórias do Instituto Oswaldo Cruz·R RuizB Alarcon de Noya
Nov 12, 2002·Memórias do Instituto Oswaldo Cruz·O NoyaH Bermúdez
Nov 12, 2002·Memórias do Instituto Oswaldo Cruz·B Alarcón de NoyaO Noya
Nov 10, 2007·Revista Da Sociedade Brasileira De Medicina Tropical·Raiza Ruiz-GuevaraOscar Noya
Feb 9, 2005·Clinical and Diagnostic Laboratory Immunology·Italo M CesariCésar Matos

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