Antigenotoxicity of probiotics and prebiotics on faecal water-induced DNA damage in human colon adenocarcinoma cells

Mutation Research
Anthony J Burns, I R Rowland

Abstract

Six strains of lactic acid producing bacteria (LAB) were incubated (1 x 10(8)cfu/ml) with genotoxic faecal water from a human subject. HT29 human adenocarcinoma cells were then challenged with the resultant samples and DNA damage measured using the single cell gel electrophoresis (comet) assay. The LAB strains investigated were Bifidobacterium sp. 420, Bifidobacterium Bb12, Lactobacillus plantarum, Streptococcus thermophilus, Lactobacillus bulgaricus and Enterococcus faecium. DNA damage was significantly decreased by all bacteria used with the exception of Strep. thermophilus. Bif. Bb12 and Lact. plantarum showed the greatest protective effect against DNA damage. Incubation of faecal water with different concentrations of Bif. Bb12 and Lact. plantarum revealed that the decrease in genotoxicity was related to cell density. Non-viable (heat treated) probiotic cells had no effect on faecal water genotoxicity. In a second study, HT29 cells were cultured in the presence of supernatants of incubations of probiotics with various carbohydrates including known prebiotics; the HT29 cells were then exposed to faecal water. Overall, incubations involving Lact. plantarum with the fructooligosaccharide (FOS)-based prebiotics Inulin, Raftilin...Continue Reading

References

Jun 1, 1991·The Journal of Applied Bacteriology·J H Cummings, G T Macfarlane
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Jan 1, 1995·Nutrition and Cancer·H AbdelaliJ F Narbonne
Jan 1, 1996·European Journal of Clinical Investigation·J SteinW F Caspary
Jan 1, 1996·Nutrition and Cancer·B L Pool-ZobelI Rowland
Jun 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BolognaniI R Rowland
Mar 6, 2002·European Journal of Nutrition·F BolognaniI R Rowland

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Citations

Jun 26, 2007·Antonie van Leeuwenhoek·Giovanna CaldiniGiovanni Cenci
Aug 19, 2007·Biotechnology Letters·Young-Seo ParkSung-Sik Yoon
Feb 4, 2006·Nature Clinical Practice. Gastroenterology & Hepatology·Janusz A Jankowski, Ernest T Hawk
Sep 27, 2013·World Journal of Microbiology & Biotechnology·Esther Swee Lan Chong
Mar 19, 2014·Research in Microbiology·Shu-Mei WangNditange Shigwedha
Dec 2, 2011·Annual Review of Food Science and Technology·Sofia Kolida, Glenn R Gibson
Aug 26, 2009·Nutrition Reviews·Jennifer R PearsonIan R Rowland
Sep 26, 2015·Regulatory Toxicology and Pharmacology : RTP·Adriana NowakAnna Otlewska
Oct 4, 2011·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·A RoesslerG Jahreis
Oct 19, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Verena Juliana KollerSiegfried Knasmüller
Jan 30, 2007·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Gabriele CapursoGianfranco Delle Fave
Feb 16, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M J McCannI R Rowland
Jul 1, 2004·Mutation Research·Andrew R Collins, Lynnette R Ferguson
Apr 5, 2016·Nutrition and Cancer·Katja T TurunenAdamantini Kyriacou
Dec 20, 2011·International Journal of Food Microbiology·Francesca TrottaGiovanni Cenci
Mar 6, 2015·Frontiers in Microbiology·Konstantinos PapadimitriouEffie Tsakalidou
Jul 11, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Adriana NowakZdzisława Libudzisz
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Leonie VogtPaul De Vos
May 10, 2005·International Journal of Cancer. Journal International Du Cancer·Chris I R GillIan Rowland
Mar 8, 2005·Nutrition and Cancer·Shusuke TodenMichael A Conlon
Jan 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jon A Vanderhoof, Rosemary Young
Jan 16, 2008·Letters in Applied Microbiology·G CenciP Bosi
May 23, 2007·Nutrition and Cancer·Chris I R GillIan Rowland
Oct 14, 2014·World Journal of Gastroenterology : WJG·Francesco RussoAntonella Orlando
Sep 27, 2019·Nature Reviews. Gastroenterology & Hepatology·Sunny H Wong, Jun Yu
Mar 4, 2020·FEMS Microbiology Letters·Natalia Garcia-GonzalezAldo Corsetti
Dec 18, 2010·Critical Reviews in Microbiology·Muhammad Irfan MasoodIkram Ullah Khan
Jul 12, 2008·Immunopharmacology and Immunotoxicology·Anna MahkonenPauli Puolakkainen

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