Abstract
A variety of biologically active products have been isolated from Gracilariopsis lemaneiformis. In the present study, two novel angiotensin-converting enzyme (ACE) inhibitory peptides, FQIN [M(O)] CILR, and TGAPCR, were screened and identified from G. lemaneiformis protein hydrolysates by LC-MS/MS. The IC50 values of FQIN [M(O)] CILR and TGAPCR were 9.64 ± 0.36 μM and 23.94 ± 0.82 μM, respectively. In the stability study, both peptides showed stabilities of pH, temperature, simulated gastrointestinal digestion, and ACE hydrolysis. The Lineweaver⁻Burk plot showed that the two peptides were noncompetitive inhibitors of ACE. Molecular docking simulated the intermolecular interactions of two peptides and ACE, and the two peptides formed hydrogen bonds with the active pockets of ACE. However, FQIN [M(O)] CILR was more closely linked to the active pockets of ACE, thereby exerting better ACE inhibition. Spontaneously hypertensive rats (SHRs) were studied with an oral dose of 10 mg/kg body weight. Both peptides reduced systolic blood pressure (SBP) and diastolic blood pressure (DBP) in SHRs, of which FQIN [M(O)] CILR was able to reduce the systolic blood pressure by 34 mmHg (SBP) (p < 0.05). Therefore, FQIN [M(O)] CILR was an excellent...Continue Reading
References
Sep 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Martin, F U Hartl
Aug 1, 1996·Journal of Dairy Science·M MaenoT Takano
Dec 22, 1999·Immunopharmacology·H Fujita, M Yoshikawa
Jan 24, 2003·Nature·Ramanathan NateshK Ravi Acharya
Jul 5, 2003·Bioscience, Biotechnology, and Biochemistry·Megumi KubaMasaaki Yasuda
Aug 23, 2005·The Journal of Nutritional Biochemistry·Huan Li, Rotimi E Aluko
Nov 9, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·G CornélissenFranz Halberg
Sep 20, 2008·Journal of Medicinal Food·Mariza FariaFlávia Maria Netto
May 19, 2010·Marine Drugs·Isuru Wijesekara, Se-Kwon Kim
Nov 3, 2010·Life Sciences·Chien-Chung ChenBeing-Sun Wung
Jul 23, 2011·Journal of Agricultural and Food Chemistry·Harsha K MarambeJanitha P D Wanasundara
May 1, 2012·European Journal of Medicinal Chemistry·A C RohitH S Aparna
May 8, 2014·International Journal of Inflammation·Maricica PacurariKenneth Ndebele
May 20, 2014·Food Chemistry·Elizabeth EscuderoFidel Toldrá
May 29, 2014·Journal of Agricultural and Food Chemistry·Xiang DuanXueming Xu
Aug 12, 2014·Peptides·Qiongying WuZhongzheng Gui
Oct 13, 2014·Food Chemistry·Rafik BaltiNaima Nedjar-Arroume
Apr 18, 2015·Annual Review of Food Science and Technology·Rotimi E Aluko
Jan 19, 2016·Food Chemistry·Qiongying WuCong Kuang
Feb 11, 2016·Marine Drugs·Tomoe FurutaHideki Kishimura
Jun 11, 2016·Food Chemistry·Saikun PanDongrui Yao
Oct 18, 2016·Oncology Reports·Peizhen LiShengbin Li
Dec 17, 2016·Food Chemistry·Patricia García-MoraCristina Martinez-Villaluenga
Sep 11, 2017·Food Research International·Min JinRunying Zeng
Dec 21, 2017·3 Biotech·Shengliang YuanSheng Wang
Dec 31, 2017·Food Chemistry·Chunlei LiuHongmei Li
Jan 19, 2018·Journal of Agricultural and Food Chemistry·Jingli XieDongzhi Wei
Mar 10, 2018·Journal of Agricultural and Food Chemistry·Kai LinDayou Cheng
Citations
Aug 9, 2019·Current Drug Targets·Lakshmanan LoganathanKarthikeyan Muthusamy
Jun 11, 2020·Natural Product Reports·Fatma H Al-Awadhi, Hendrik Luesch
Jul 7, 2020·Electrophoresis·Maria CermeñoRichard J FitzGerald
Nov 30, 2018·Marine Drugs·Shan-Shan ZhangKe-Chun Liu
Aug 11, 2019·Marine Drugs·Pengru LiuLixia Sun
Aug 31, 2019·Marine Drugs·Tatiana V Ovchinnikova
Aug 23, 2020·Marine Drugs·Milica PavlicevicMarta Marmiroli
Feb 6, 2020·PloS One·Rana Adnan TahirSheikh Arslan Sehgal
Dec 15, 2020·Archives of Animal Nutrition·Tânia Zóia MiltenburgRicardo Souza Vasconcellos
Oct 16, 2019·Peptides·Bingjun QianOscar D Villarreal
Feb 18, 2021·Journal of Agricultural and Food Chemistry·Yanbo HuangSong-Qing Hu
Jun 1, 2021·Journal of Food Science·Guilan YangWenjun Li
Nov 17, 2021·Food & Function·Junbo ChenQiyi He
Jan 28, 2022·Food & Function·Yun-Tao ZhaoShucheng Liu