Antiinflammatory effects of amniotic membrane transplantation in ocular surface disorders

Cornea
S ShimmuraK Tsubota

Abstract

To determine whether the sequestration of inflammatory cells plays a role in the antiinflammatory effects of amniotic membrane transplantation to the ocular surface. Amniotic membrane grafts were prepared from placental tissue procured from mothers undergoing planned Cesarean sections. A detailed explanation was given to all donors, and a written consent was obtained before processing. Amniotic membrane tissue was dissected into 3- x 3-cm segments, rinsed in phosphate buffered saline, and stored in dimethyl sulfoxide solutions at -80 degrees C until use. In a clinical series, amniotic membrane patches of the ocular surface were performed in 20 eyes of 20 patients with persistent corneal epithelial defects, or as a prophylactic measure after corneal limbal transplantation. Amniotic membrane patches were harvested after a 1-week observation period and were subjected to histopathologic examinations by hematoxylin and eosin staining. Inflammatory cells trapped within the amniotic membrane were labeled by immunocytochemistry using anti-CD14, CD4, CD8, and CD20 antibodies. TUNEL (TdT-mediated dUTP nick end labeling) staining was done to observe cells undergoing apoptosis. The T cell line Molt 4 was co-cultured with amniotic membrane ...Continue Reading

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References

Aug 1, 1979·American Journal of Obstetrics and Gynecology·J D Trelford, M Trelford-Sauder
Aug 15, 1978·American Journal of Obstetrics and Gynecology·B J van HerendaelI Brosens
Mar 1, 1997·American Journal of Ophthalmology·S H Lee, S C Tseng
Dec 24, 1997·American Journal of Ophthalmology·S C TsengS H Lee
Jun 3, 1999·The New England Journal of Medicine·K TsubotaJ Shimazaki
Jun 1, 1946·The British Journal of Ophthalmology·A SORSBY, H M SYMONS

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Citations

Aug 13, 2008·Archives of Ophthalmology·Ahmad KheirkhahScheffer C G Tseng
Dec 29, 2004·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·B SeitzF E Kruse
May 18, 2013·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·K EngelmannC Werner
Sep 20, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Juan YeKe Yao
Dec 12, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Azusa Nagai-KusuharaAkira Negi
Aug 21, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Henning ThomasenDaniel Meller
May 18, 2012·Cell and Tissue Research·A C MamedeM F Botelho
Feb 14, 2007·Japanese Journal of Ophthalmology·Hiroshi TakahashiMasamichi Ishizaki
Sep 18, 2009·Veterinary Research Communications·Marissak KalpravidhSudson Sirivaidyapong
Nov 12, 2003·Survey of Ophthalmology·Martin GrueterichScheffer C G Tseng
Jan 9, 2004·Survey of Ophthalmology·Harminder S DuaV Senthil Maharajan
Apr 30, 2003·American Journal of Ophthalmology·Shigeto ShimmuraKazuo Tsubota
Sep 10, 2003·Transplantation Proceedings·O GrisA Adán
Mar 22, 2003·Journal of Cataract and Refractive Surgery·Edgar M EspanaScheffer C G Tseng
Sep 17, 2002·Journal of the American College of Surgeons·Michael A Lemp
Jan 1, 2004·Cornea·Michelle K Rhee, V Vinod Mootha
Nov 30, 2006·Cornea·Raja NarayananM Cristina Kenney
Aug 28, 2007·Cornea·Ching-Long ChenDa-Wen Lu
Sep 23, 2009·Cornea·Hosam ShehaScheffer C G Tseng
Jul 11, 2008·Investigative Ophthalmology & Visual Science·Hua HeScheffer C G Tseng
Oct 14, 2011·BMC Complementary and Alternative Medicine·Fadhilah Zainal AbidinNorzana Abd Ghafar
Jun 15, 2007·Journal of Medical Case Reports·Tetsuya KawakitaJun Shimazaki
Jun 2, 2012·Clinical Ophthalmology·Ayse Yagci
Dec 4, 2012·Indian Journal of Ophthalmology·Ozlem Yalcin TokFirdevs Ornek
Dec 18, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Alessandra MiceraStefano Bonini
May 1, 2013·Regenerative Medicine·Bakiah ShaharuddinAnnette Meeson

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