Antileishmanial activity and evaluation of the mechanism of action of strychnobiflavone flavonoid isolated from Strychnos pseudoquina against Leishmania infantum

Parasitology Research
Paula Sousa LageEduardo A F Coelho

Abstract

The present study aimed to investigate the in vitro antileishmanial activity of strychnobiflavone flavonoid against Leishmania infantum, as well as its mechanism of action, and evaluate the ex vivo biodistribution profile of the flavonoid in naive BALB/c mice. The antileishmanial activity (IC50 value) of strychnobiflavone against stationary promastigote and amastigote-like stages of the parasites was of 5.4 and 18.9 μM, respectively; with a 50% cytotoxic concentration (CC50) value of 125.0 μM on murine macrophages, resulting in selectivity index (SI) of 23.2 and 6.6, respectively. Amphotericin B, used as a positive control, presented SI values of 7.6 and 3.3 for promastigote and amastigote-like stages of L. infantum, respectively. The strychnobiflavone was also effective in reducing in significant levels the percentage of infected macrophages, as well as the number of amastigotes per macrophage, after the treatment of infected macrophages using the flavonoid. By using different fluorescent probes, we investigated the bioenergetics metabolism of L. infantum promastigotes and demonstrated that the flavonoid caused the depolarization of the mitochondrial membrane potential, without affecting the production of reactive oxygen speci...Continue Reading

References

Oct 1, 1991·Experimental Parasitology·P S DoyleD M Dwyer
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·R Lainson
Feb 1, 1996·Journal of the American Academy of Dermatology·S A Grevelink, E A Lerner
Apr 1, 1997·Clinical Microbiology Reviews·J AlvarJ Moreno
Dec 13, 2000·International Journal for Parasitology·R W Ashford
Apr 17, 2001·Antimicrobial Agents and Chemotherapy·J C DelorenziE M Saraiva
Nov 13, 2001·International Journal of Experimental Pathology·H W Murray
Jul 16, 2003·Journal of Ethnopharmacology·V F Andrade-NetoA U Krettli
Jul 26, 2003·Journal of Natural Products·David J NewmanKenneth M Snader
Oct 16, 2003·Phytochemistry·Piyanuch ThongphasukRobert Verpoorte
Oct 29, 2003·Trends in Parasitology·Simon L Croft, Graham H Coombs
Dec 31, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sujit K BhattacharyaJonathan D Berman
Feb 26, 2004·Antimicrobial Agents and Chemotherapy·Maria Mamani-MatsudaM Djavad Mossalayi
May 15, 2004·Research in Microbiology·Luciana FumarolaOlga Brandonisio
Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Geneviève PhilippeMichel Frédérich
Aug 27, 2004·The Journal of Antimicrobial Chemotherapy·Ricardo M Santa-RitaSolange L de Castro
Dec 24, 2004·Antimicrobial Agents and Chemotherapy·Tatiana Shioji TiumanCelso Vataru Nakamura
Jul 13, 2005·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L G RochaJ M Barbosa-Filho
Dec 14, 2005·Parasitology International·Tânia Ueda-NakamuraCelso Vataru Nakamura
Aug 31, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Caryn BernJorge Alvar
Feb 14, 2007·Journal of Ethnopharmacology·Edison OsorioSara Robledo
Apr 24, 2007·Travel Medicine and Infectious Disease·Philippe Minodier, Philippe Parola
Oct 17, 2007·Nature Reviews. Microbiology·François ChappuisMarleen Boelaert
Apr 5, 2008·Parasitology Research·Dilvani O SantosHelena C Castro
Oct 2, 2008·Current Opinion in Clinical Nutrition and Metabolic Care·Stephan C Bischoff
Jan 23, 2009·The Journal of Antimicrobial Chemotherapy·Claudio AnnaloroGiorgio Lambertenghi Deliliers
Apr 3, 2009·Bioorganic & Medicinal Chemistry Letters·Tanvir KhaliqT Narender
Apr 22, 2009·The International Journal of Biochemistry & Cell Biology·Wanderley de SouzaJuliany C F Rodrigues
Jun 6, 2009·Journal of Natural Products·Clotilde MarínManuel Sánchez-Moreno
Jun 9, 2009·Free Radical Biology & Medicine·Rubem F S Menna-BarretoSolange L de Castro
Sep 29, 2009·International Journal of Antimicrobial Agents·Piyali MukherjeeBanasri Hazra
Jan 13, 2010·Parasitology International·Michele Cristina VendramettoTânia Ueda-Nakamura
Jan 22, 2010·The Journal of Antimicrobial Chemotherapy·Karin SeifertSimon L Croft
May 11, 2010·PLoS Neglected Tropical Diseases·Jair L Siqueira-NetoLucio H Freitas-Junior
Sep 15, 2010·Antimicrobial Agents and Chemotherapy·Luis CarvalhoFrancisco Gamarro

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Citations

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May 23, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Miguel Angel Chávez-FumagalliEduardo Antonio Ferraz Coelho
Feb 26, 2019·PLoS Neglected Tropical Diseases·Christine Achiaa AntwiTheresa Manful Gwira
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