Antileukemic effect of zerumbone-loaded nanostructured lipid carrier in WEHI-3B cell-induced murine leukemia model

International Journal of Nanomedicine
Heshu Sulaiman RahmanHasina Begum

Abstract

Cancer nanotherapy is progressing rapidly with the introduction of many innovative drug delivery systems to replace conventional therapy. Although the antitumor activity of zerumbone (ZER) has been reported, there has been no information available on the effect of ZER-loaded nanostructured lipid carrier (NLC) (ZER-NLC) on murine leukemia cells. In this study, the in vitro and in vivo effects of ZER-NLC on murine leukemia induced with WEHI-3B cells were investigated. The results from 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, Hoechst 33342, Annexin V, cell cycle, and caspase activity assays showed that the growth of leukemia cells in vitro was inhibited by ZER-NLC. In addition, outcomes of histopathology, transmission electron microscopy, and Tdt-mediated dUTP nick-end labeling analyses revealed that the number of leukemia cells in the spleen of BALB/c leukemia mice significantly decreased after 4 weeks of oral treatment with various doses of ZER-NLC. Western blotting and reverse-transcription quantitative polymerase chain reaction assays confirmed the antileukemia effects of ZER-NLC. In conclusion, ZER-NLC was shown to induce a mitochondrial-dependent apoptotic pathway in murine leukemia. Loading of ZER in NL...Continue Reading

Citations

Oct 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katayoon KalantariRosfarizan Mohamad
Feb 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sosmitha GirisaAjaikumar B Kunnumakkara
Jun 18, 2019·Current Medicinal Chemistry·Carmelo PugliaElisabetta Esposito
Apr 30, 2020·International Journal of Nanomedicine·Heshu Sulaiman RahmanNoorjahan Banu Alitheen
Jul 10, 2020·Chemistry & Biodiversity·Luiza Stolz CruzFlávio Luís Beltrame
Jun 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Noraini NordinNoorjahan Banu Alitheen
Sep 15, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Hemn OthmanRasedee Abdullah
Aug 25, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Tareq Abu-IzneidKannan R R Rengasamy

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BETA
X-ray
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chips
light microscopy
protein assay
PCR
electrophoresis
flow cytometry

Software Mentioned

Summit
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SPSS

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