Antiliver Fibrosis Screening of Active Ingredients from Apium graveolens L. Seeds via GC-TOF-MS and UHPLC-MS/MS

Evidence-based Complementary and Alternative Medicine : ECAM
Ming QiaoJunping Hu

Abstract

Although several studies have been performed on Apium graveolens L.(celery) seeds, their antiliver fibrosis effects remain to be unexplored. Firstly, we detected the effects of celery seeds extracted with different concentrations of aqueous ethanol on the proliferation of HSC-LX2 cells. Then, we detected the effects of fractions of the optimal effect extract on the proliferation and apoptosis of HSC-LX2 cells. Finally, the compounds of petroleum ether (PP), ethyl acetate (PE), n-butyl alcohol (PB), and water fractions (PW) of the optimal effect extract were determined by GC-TOF-MS and UHPLC-MS/MS, to confirm the potentially antifibrotic compounds combined with pharmacodynamic experiment of monomer compounds in vitro. The results revealed that 60% ethanol extract of celery seeds (60-extract) exhibited remarkable inhibition effect on the proliferation of HSC-LX2 cells compared with 95% ethanol and aqueous extract. Besides, it validated that the inhibition rates of PP, PE, PB, and PW on the proliferation of HSC-LX2 cells were 75.14%, 73.52%, 54.09%, and 43.36%, and their percentage of apoptotic cells were 37.5%, 4.3%, 0.7%, and 0.1% at high doses, respectively. Additionally, it was manifested that apigenin, aesculetin, and butylph...Continue Reading

References

Jul 15, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Zhixia DongJianhua Wang
Oct 5, 2016·Middle East Journal of Digestive Diseases·Hedyeh EbrahimiAmir Ali Sohrabpour
Jan 5, 2017·British Journal of Pharmacology·Feng ZhangShizhong Zheng
Jan 28, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Fujun YuJianjian Zheng
Feb 6, 2017·Biochemical and Biophysical Research Communications·Panpan XiongFei Liu
May 17, 2017·Advanced Drug Delivery Reviews·Takaaki HigashiYujin Hoshida
Jun 1, 2017·Seminars in Liver Disease·Fernando AlegreAriel E Feldstein
Jul 14, 2017·Journal of Evidence-based Complementary & Alternative Medicine·Wesam Kooti, Nahid Daraei
Mar 22, 2018·BMC Complementary and Alternative Medicine·Pennapa ChonpathompikunlertMorakot Sroyraya
Apr 1, 2018·Naunyn-Schmiedeberg's Archives of Pharmacology·Huiliang QiuChunhua Ding
Apr 14, 2018·Plant Physiology and Biochemistry : PPB·Jie-Xia LiuAi-Sheng Xiong
Apr 27, 2018·Journal of Extracellular Vesicles·Li ChenDavid R Brigstock
Jun 2, 2018·Journal of Controlled Release : Official Journal of the Controlled Release Society·Jian-Bin QiaoHu-Lin Jiang
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhaoguo LiuWei Zhang
Jul 19, 2018·Preventive Nutrition and Food Science·Karim DolatiHamid R Sadeghnia
Sep 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Siwei TanBin Wu
Nov 1, 2018·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yuping ZhouJunming Guo
Dec 26, 2018·Journal of Hepatology·Hsin-Chieh TsayAmar Deep Sharma
May 31, 2019·Journal of Cellular and Molecular Medicine·Wei WangJianjian Zheng
Jul 22, 2019·The Journal of Nutritional Biochemistry·Yanan LvLiping Yan
Oct 1, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Guangyao LiYuanjia Hu
Oct 23, 2019·Life Sciences·Mostafa M SalahSalama A Salama

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
flow cytometry

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis