Antimicrobial action of oleanolic acid on Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis

PloS One
Sejeong KimKyoung-Hee Choi

Abstract

This study investigated the antimicrobial action of oleanolic acid against Listeria monocytogenes, Enterococcus faecium, and Enterococcus faecalis. To determine the cytotoxicity of oleanolic acid, HEp-2 cells were incubated with oleanolic acid at 37°C. MICs (minimal inhibition concentrations) for L. monocytogenes, E. faecium, and E. faecalis were determined using two-fold microdilutions of oleanolic acid, and bacterial cell viability was then assessed by exposing the bacteria to oleanolic acid at 2 × MIC. To investigate the mode of antimicrobial action of oleanolic acid, we measured leakage of compounds absorbing at 280 nm, along with propidium iodide uptake. Scanning electron microscope (SEM) images were also analysed. The viability of HEp-2 cells decreased (P < 0.05) at oleanolic acid concentrations greater than 128 μg mL(-1). The MICs were 16-32 μg mL(-1) for L. monocytogenes and 32-64 μg mL(-1) for E. faecium and E. faecalis, and bacterial cell viability decreased (P < 0.05) about 3-4 log CFU mL(-1) after exposure to 2 × MIC of oleanolic acid. Leakage of 280 nm absorbing materials and propidium iodide uptake was higher in oleanolic acid -treated cells than in the control. The cell membrane was damaged in oleanolic acid-trea...Continue Reading

References

Dec 1, 1991·Antimicrobial Agents and Chemotherapy·B L LeeH F Chambers
Oct 1, 1994·Clinical Microbiology Reviews·B D JettM S Gilmore
Mar 12, 1998·Infectious Disease Clinics of North America·B M Allos
Mar 29, 2000·Journal of Applied Microbiology·S D CoxS G Wyllie
Jun 29, 2002·Applied and Environmental Microbiology·Robert GelsominoJ Swings
Nov 5, 2003·International Journal of Food Microbiology·Lucila SaavedraGraciela Font de Valdez
Jul 5, 2005·International Journal of Food Microbiology·Andreja Canzek MajhenicBogdan Perko
Oct 17, 2006·The Journal of Antimicrobial Chemotherapy·G L French
Jan 24, 2007·Journal of Ethnopharmacology·Adelina Jiménez-ArellanesJulieta Luna-Herrera
Aug 28, 2007·Microbes and Infection·Bala Swaminathan, Peter Gerner-Smidt
Oct 7, 2008·Journal of Ethnopharmacology·Stéphane FontanayRaphaël Emmanuel Duval
Nov 7, 2009·Antonie van Leeuwenhoek·Anna KurekMagdalena Popowska
Nov 10, 2009·International Journal of Medical Microbiology : IJMM·Guoqing XiaAndreas Peschel
Dec 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sheng-lei YanMei-chin Yin
Jan 27, 2010·Chemico-biological Interactions·Xin WangChun-Xu Hai
Apr 30, 2010·European Journal of Medicinal Chemistry·Mallepally V ReddySurrinder Koul
Dec 6, 2012·Journal of Food Protection·Ebrahim RahimiHussein Farzinezhadizadeh
Feb 1, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Amit Kumar TyagiPetar D Marin

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Citations

Nov 16, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Taiwo Betty AyelesoEmmanuel Mukwevho
Nov 28, 2018·Korean Journal for Food Science of Animal Resources·In-Chan HwangDae-Kyung Kang
Jul 23, 2020·Natural Product Research·Zareena KiranErum Iqbal
Nov 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rebecca BorellaMarcello Pinti
Sep 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vuyolwethu KhwazaBlessing A Aderibigbe
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aurita ButkeviciuteValdimaras Janulis
Oct 24, 2018·Journal of Agricultural and Food Chemistry·Yen-Ning LinYun-Ping Lim

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Methods Mentioned

BETA
FACS
flow cytometry
scanning electron microscopy

Software Mentioned

CellQuest Pro
SAS

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