Antimicrobial actions of benzimidazoles against oral streptococci

Oral Microbiology and Immunology
Phuong T M NguyenRobert E Marquis

Abstract

Benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against Helicobacter pylori. Our objective was to determine whether they are potentially useful antimicrobials against oral bacteria. Streptococcus mutans was our main test organism. It was grown in suspension cultures and biofilms. Standard physiologic assays were used to assess inhibitory actions of benzimidazoles. Benzimidazoles inhibited acid production by S. mutans in suspensions or biofilms. In pH-drop experiments, lansoprazole at a level of only 0.025 mm irreversibly inhibited acid production from glycolysis. Cell uptake of lansoprazole was found to be very pH sensitive and occurred mainly at pH values below about 5, indicating that the protonated form was taken up. Lansoprazole inhibition of glycolysis could be blocked by 2-mercaptoethanol, which suggests that disulfide bonds form between benzimidazoles and protein targets. Identified targets for benzimidazole inhibition included the phosphoenolpyruvate : sugar phosphotransferase system, the glycolytic enzymes aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase, and enzymes such...Continue Reading

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Related Concepts

Antibiotics
Cell Membrane Permeability
Enzyme Inhibitors
Glycolysis
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Hydrogen-Ion Concentration
Vestibule of Mouth
Prilosec
Phosphoenolpyruvate-Glycose Phosphotransferase System
Streptococcus

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