Antimicrobial activity of bovine NK-lysin-derived peptides on Mycoplasma bovis

PloS One
Rohana P DassanayakeTimothy A Reinhardt

Abstract

Antimicrobial peptides (AMPs) are a diverse group of molecules which play an important role in the innate immune response. Bovine NK-lysins, a type of AMP, have been predominantly found in the granules of cytotoxic T-lymphocytes and NK-cells. Bovine NK-lysin-derived peptides demonstrate antimicrobial activity against various bacterial pathogens, including several involved in bovine respiratory disease complex (BRDC) in cattle; however, such studies are yet to be performed with one important contributor to the BRDC, Mycoplasma bovis. Therefore, the goal of this study was to assess the antimicrobial activity of bovine NK-lysin-derived peptides on M. bovis. Thirty-mer synthetic peptides corresponding to the functional region helices 2 and 3 of bovine NK-lysins NK1, NK2A, NK2B, and NK2C were evaluated for killing activity on M. bovis isolates. Among four peptides, NK2A and NK2C showed the highest antimicrobial activity against the M. bovis isolates tested. All four NK-lysin peptides induced rapid plasma membrane depolarization in M. bovis at two concentrations tested. However, based on propidium iodide uptake, only NK2A and NK2C appeared capable of causing structural damage to M. bovis plasma membrane. Confocal microscopy, flow cyt...Continue Reading

References

Jan 1, 1995·Annual Review of Immunology·H G Boman
Jan 1, 1993·Sub-cellular Biochemistry·J Dahl
Nov 1, 1996·Veterinary Immunology and Immunopathology·M AnderssonG H Gudmundsson
Dec 11, 2002·Trends in Parasitology·Jacopo Vizioli, Michel Salzet
Jan 25, 2003·Antimicrobial Agents and Chemotherapy·Thomas JacobsMatthias Leippe
Apr 1, 2003·Animal Health Research Reviews·Samithamby JeyaseelanSamuel K Maheswaran
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Janice J EndsleyD Mark Estes
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
May 30, 2006·Current Microbiology·Koichi KuwanoYutaka Kida
Apr 5, 2007·Nature Protocols·Norma J Greenfield
Jun 30, 2007·Biochimica Et Biophysica Acta·Raquel F EpandRichard M Epand
Jan 26, 2008·Animal Health Research Reviews·Jeff L Caswell, Marie Archambault
Mar 12, 2008·Antimicrobial Agents and Chemotherapy·Christoph GelhausMatthias Leippe
May 8, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Neil DyerEvelyn Schamber
Apr 15, 2009·Antimicrobial Agents and Chemotherapy·Ilknur SenyürekBirgit Schittek
Nov 20, 2009·Nucleic Acids Research·Shaini ThomasSusan Idicula-Thomas
Dec 17, 2009·Animal Health Research Reviews·Anthony W Confer
Jun 30, 2010·Antimicrobial Agents and Chemotherapy·Raquel F EpandRichard M Epand
Jul 13, 2011·Journal of Veterinary Internal Medicine·F P MaunsellE D Janzen
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Marty K SoehnlenBhushan M Jayarao
Nov 15, 2011·Critical Reviews in Biotechnology·Mukesh PasupuletiMartin Malmsten
Jul 24, 2012·Frontiers in Immunology·Jeffrey R BrenderAyyalusamy Ramamoorthy
Aug 1, 2012·Journal of Lipids·Jonathan D Kornspan, Shlomo Rottem
Jul 5, 2013·PloS One·Xiaowei ZhaoQingshan Huang
Jul 13, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Karen B RegisterM Claire Windeyer
Sep 3, 2013·FEBS Letters·Ho Jin ParkIn Hee Lee
Dec 1, 2014·Veterinary Microbiology·Karen B RegisterF Chris Minion
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Junfeng ChenJames E Womack
Feb 18, 2017·Animal : an International Journal of Animal Bioscience·B EarleyS Gupta

❮ Previous
Next ❯

Citations

Apr 25, 2020·The Veterinary Clinics of North America. Food Animal Practice·Jodi L McGill, Randy E Sacco

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stress
flow cytometry
transmission electron microscopy
Circular dichroism
confocal microscopy

Software Mentioned

Elements Advanced Research
Adobe Photoshop Elements
NIS
Excel
FlowJo
Peptide

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.