Antimicrobial Activity of ILTI, a Kunitz-Type Trypsin Inhibitor from Inga laurina (SW.) Willd

Current Microbiology
Maria L R MacedoSimone Maria-Neto

Abstract

Over the last few years, a growing number of proteinase inhibitors have been isolated from plants and particularly from seeds and have shown antimicrobial activity. A 20,000 Da serine peptidase inhibitor, named ILTI, was isolated from Inga laurina seeds and showed potent inhibitory enzymatic activity against trypsin. The aim of this study was to determine the effects of ILTI on the growth of pathogenic and non-pathogenic microorganisms. We observed that ILTI strongly inhibited in particular the growth of Candida tropicalis and Candida buinensis, inducing cellular agglomeration. However, it was ineffective against human pathogenic bacteria. We also investigated the potential of ILTI to permeabilize the plasma membrane of yeast cells. C. tropicalis and C. buinensis were incubated for 24 h with the ILTI at different concentrations, which showed that this inhibitor induced changes in the membranes of yeast cells, leading to their permeabilization. Interestingly, ILTI induced the production of reactive oxygen species (ROS) in C. tropicalis and C. buinensis cells. Finally, ILTI was coupled with fluorescein isothiocyanate, and subsequent treatment of C. tropicalis and C. buinensis with DAPI revealed the presence of the labeled protein...Continue Reading

References

Dec 3, 1999·Applied and Environmental Microbiology·K ThevissenW F Broekaert
Dec 26, 2001·Nucleic Acids Research·F De LeoL R Ceci
Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Michel MonodOlivier Jousson
Dec 1, 1952·Journal of Bacteriology·A POMALES-LEBRON, C FERNANDEZ
May 7, 2004·Journal of Plant Physiology·José A CasarettoLuis J Corcuera
Oct 7, 2004·Archives of Biochemistry and Biophysics·Soghra Khatun HaqRizwan Hasan Khan
Apr 6, 2005·Biochemical and Biophysical Research Communications·Jin-Young KimKyung-Soo Hahm
Jun 22, 2006·Current Protein & Peptide Science·Alfonso Clementea, C Domoney
Jun 19, 2007·Toxicon : Official Journal of the International Society on Toxinology·Suzanna F F RibeiroValdirene M Gomes
Jul 8, 2009·International Journal of Molecular Sciences·Jin-Young KimYoonkyung Park
Apr 7, 2010·Biochimie·Maria Luiza V OlivaMisako U Sampaio
Nov 26, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Maria Lígia Rodrigues MacedoCaio Fernando Ramalho de Oliveira
Apr 28, 2011·Journal of Natural Products·Thaís B LimaOctávio L Franco
Dec 14, 2011·Antonie van Leeuwenhoek·Suzanna F F RibeiroValdirene M Gomes
Aug 14, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Vanessa da S RamosMaria L R Macedo
Mar 13, 2013·Sub-cellular Biochemistry·Dorte FreesHanne Ingmer

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Citations

Sep 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xixi CaiShaoyun Wang
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
Jan 7, 2019·International Journal of Biological Macromolecules·Mauricio V CruzKátia F Fernandes
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Karina Margareti Alencar de BarrosMaria Lígia Rodrigues Macedo

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