Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus.

Stem Cell Research & Therapy
Haruyo YagiRocky S Tuan

Abstract

There have been limited advances in the treatment of bone and joint infections, which currently involves a combination of surgery and antibiotic administration. There is a timely need in orthopedics to develop more effective and less invasive forms of antimicrobial prophylaxis and treatment. The antibacterial effect of adult tissue-derived mesenchymal stem cells (MSCs) has recently been investigated against Escherichia coli and Staphylococcus aureus. The main mechanism of action is postulated to be via MSC production of the cationic antimicrobial peptide, LL-37. This study examines the antimicrobial activity of adipose-derived human MSCs (ASCs) on S. aureus, specifically examining the role of LL-37 and regulation of its expression. Bacteria colony-forming unit (CFU) assay was used to assess antimicrobial activity. Our results showed that the ASC-conditioned medium significantly inhibited the growth of S. aureus under standard culture conditions with or without the continued presence of ASCs. Also, the treatment of ASCs with 1,25-dihydroxy vitamin D3 elevated LL-37 expression and enhanced their antimicrobial activity. In support, treatment with the vitamin D receptor inhibitor, GW0742, blocked the antimicrobial activity of ASCs....Continue Reading

References

May 1, 2004·The Journal of Antimicrobial Chemotherapy·Elizabeth S R Darley, Alasdair P MacGowan
Oct 20, 2004·Journal of Cellular and Molecular Medicine·D BakshR S Tuan
Jun 29, 2005·The Journal of Investigative Dermatology·Belén López-GarcíaRichard L Gallo
Jul 28, 2005·Journal of Inflammation·Jennifer M RyanBernard P Mahon
Oct 11, 2005·Biotechnology Letters·Kris De Smet, Roland Contreras
Jul 14, 2007·Current HIV Research·Peter BergmanJohan Söderlund
Mar 4, 2008·The Journal of Bone and Joint Surgery. American Volume·Patrick BirminghamRocky S Tuan
Jul 2, 2008·Infection and Immunity·Joerg OverhageRobert E W Hancock
Dec 17, 2008·Current Opinion in Hematology·Anastasia Nijnik, Robert E W Hancock
Mar 11, 2009·Clinical Pharmacokinetics·Cornelia B LandersdorferFritz Sörgel
Nov 28, 2009·Current Stem Cell Research & Therapy·Alexander M BaileyAdam J Katz
Apr 15, 2010·Clinical Orthopaedics and Related Research·Leo A WhitesideMarcel E Roy
Jul 14, 2010·Clinical Orthopaedics and Related Research·Farshid GuilakJeffrey M Gimble
Jan 26, 2011·Nature Reviews. Endocrinology·Martin Hewison
Sep 20, 2011·The Journal of Trauma·Clinton K MurrayUNKNOWN Prevention of Combat-Related Infections Guidelines Panel
Aug 10, 2011·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Alexander Y HuiRobert L Sah
Oct 26, 2011·Current Stem Cell Research & Therapy·Baljinder S Dhinsa, Adetola B Adesida
Mar 20, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anna KrasnodembskayaMichael A Matthay
Aug 25, 2012·Future Medicinal Chemistry·Andreas S Jacobsen, Håvard Jenssen
Jan 8, 2013·American Journal of Respiratory and Critical Care Medicine·Jae W LeeMichael A Matthay
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Mahmoud H Abou AlaiwaMichael J Welsh
Nov 12, 2015·Biochimica Et Biophysica Acta·Daniela XhindoliAlessandro Tossi
Dec 1, 2014·Journal of Experimental Orthopaedics·Brian D MarianiRocky S Tuan
Mar 18, 2016·Postȩpy dermatologii i alergologii·Małgorzata Marcinkiewicz, Sławomir Majewski
Nov 1, 2016·Archivum Immunologiae Et Therapiae Experimentalis·Anna BajekTomasz Drewa
Oct 4, 2016·Biotechnology Advances·Samit Kumar NandiBiswanath Kundu
Dec 30, 2016·Reviews in Endocrine & Metabolic Disorders·Harjit Pal BhattoaPawel Pludowski
Sep 20, 2017·Journal of Cellular Physiology·Debora Lo FurnoRosario Giuffrida
Oct 28, 2017·Stem Cells Translational Medicine·Rebekah M SamsonrajSimon M Cool
Feb 21, 2018·The International Journal of Lower Extremity Wounds·Konstantinos MarkakisFrank L Bowling

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