Antimicrobial activity of platelet (PLT)-poor plasma, PLT-rich plasma, PLT gel, and solvent/detergent-treated PLT lysate biomaterials against wound bacteria

Transfusion
Thierry BurnoufLin-Wen Lee

Abstract

Platelet (PLT) gels exhibit antimicrobial activity useful for wound healing. The nature of the antibacterial component(s) is unknown. PLT-poor plasma (PPP), PLT-rich plasma (PRP), PLT gel (PG), and solvent/detergent-treated PLT lysate (S/D-PL) from two donors were evaluated either native or after complement heat inactivation. Materials were spiked at a 10% ratio (vol/vol) with approximately 10(7-8) colony-forming units/mL with four Gram-positive and four Gram-negative bacteria of the wound flora. Bacterial count was determined by plate assays at time of spiking and after 3 and 48 hours at 31°C. Bacteria growth inhibition tests were also performed. There was no viable Escherichia coli colony for 48 hours after spiking to the plasma and PLT materials from both donors, corresponding to greater than 7.51 to greater than 9.05 log inactivation. Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus were inactivated (approx. 4.7, 7, and 2 log, respectively) 3 hours after spiking to PRP, PPP, or S/D-PL from the first donor but less (1.1, 4.6, and 0.2 log, respectively) in PG, before a regrowth at 48 hours in all materials. Similar data were obtained with the second donor. No plasma and PLT material had antimicrobial a...Continue Reading

References

Jan 1, 1984·Annual Review of Immunology·K A JoinerM M Frank
Jan 1, 1994·The Journal of Investigative Dermatology·V FalangaA Moshell
Jan 1, 1993·Annual Review of Immunology·S H Kaufmann
Oct 1, 1995·Trends in Microbiology·J TravisH Maeda
Dec 1, 1995·Burns : Journal of the International Society for Burn Injuries·H Vindenes, R Bjerknes
Jun 1, 1997·The Surgical Clinics of North America·M C Robson
Dec 24, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M R Yeaman
Oct 19, 2001·Transfusion·E L Snyder, B C Calhoun
Nov 20, 2002·Infection and Immunity·Yi-Quan TangMichael E Selsted
Mar 17, 2004·Current Opinion in Infectious Diseases·Ruth Edwards, Keith G Harding
Mar 16, 2007·The Journal of Bone and Joint Surgery. British Volume·T M BieleckiT Wielkoszynski
Jul 5, 2007·Journal of Clinical Microbiology·Diane M CitronMurray A Abramson
Jul 12, 2007·Seminars in Immunopathology·Katherine Radek, Richard Gallo
Aug 19, 2007·Nature Reviews. Microbiology·Michael R Yeaman, Nannette Y Yount
Oct 26, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Dirk Jan F MoojenWouter J A Dhert
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sean M O'ConnellHerbert Dardik
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Stephan BarrientosMarjana Tomic-Canic
May 20, 2009·Blood Reviews·Price Blair, Robert Flaumenhaft
Jun 23, 2009·Burns : Journal of the International Society for Burn Injuries·Norbert PalluaMarta Markowicz
Sep 11, 2009·Journal of Thrombosis and Haemostasis : JTH·D Cox
Mar 2, 2010·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Tim Mo ChenThierry Burnouf
Apr 8, 2010·Critical Reviews in Immunology·Mohamed R Daha
Mar 1, 2011·Biologicals : Journal of the International Association of Biological Standardization·N GreppiM Marconi
Nov 19, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Sashwati RoyChandan K Sen

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Citations

May 7, 1998·Transfusion Science·L A Harker
Apr 23, 2013·BioMed Research International·Yuan-Sheng TzengThierry Burnouf
Jan 5, 2014·PloS One·Maria Chiara BarsottiGiorgio Soldani
Jan 1, 2014·Journal of Thrombosis and Thrombolysis·Yuanxi XuHongmin Sun
Apr 25, 2014·Sports Medicine·Taralyn M McCarrelLisa A Fortier
Dec 5, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Giuseppina SandriCarla Caramella
Nov 26, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Hsiu-Chen LinThierry Burnouf
Apr 15, 2014·Transfusion·Marie-Pierre CayerLouis Thibault
Aug 12, 2014·Cytotherapy·Erminia MarianiAndrea Facchini
Jul 10, 2013·International Wound Journal·Fabio NicoliValerio Cervelli
Aug 7, 2014·Biologicals : Journal of the International Association of Biological Standardization·W A GermishuizenT Burnouf
Aug 1, 2015·BMC Microbiology·Erminia MarianiGiuseppe Filardo
Dec 30, 2014·Transfusion Medicine Reviews·Maria Rosaria De PascaleClaudio Napoli
Dec 3, 2016·The American Journal of Sports Medicine·Matthias KiebThomas Tischer
Oct 2, 2017·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·R A CetinkayaA Gunal
Oct 2, 2019·International Wound Journal·Beatriz G R B de OliveiraBruna M F B Pires
Sep 13, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yan GaoHao Chen
Sep 24, 2020·International Journal of Molecular Sciences·Isabel AndiaNicola Maffulli
Mar 18, 2016·Clinical and Experimental Dental Research·Kazuhiko NishiyamaTomoyuki Kawase
Jan 25, 2020·Scientific Reports·Aref ShariatiEhsanollah Ghaznavi-Rad
Dec 16, 2017·Journal of Wound Care·V BabaeiS Teimourian
Jul 28, 2018·BioMed Research International·Agata Cieślik-BieleckaPaweł Reichert
Mar 13, 2018·Frontiers in Medicine·Victor J DrewThierry Burnouf
Jan 11, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jessica M GilbertieLauren V Schnabel
Nov 28, 2020·International Journal of Oral Science·Mengge FengYufeng Zhang

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