Antimicrobial activity of puffball(Bovistella radicata) and separation of bioactive compounds

AMB Express
Yong YeQingmei Zeng

Abstract

To test the antimicrobial activity of different extracts and fermentation broth from puffball(Bovistella radicata), the different extracts and fermentation broth of puffball were prepared, the active fraction was investigated by UPLC-UV-MS and semi-preparative chromatograph. Through zones of inhibition (ZOI) and minimum inhibitory concentrations (MIC) tests, the supernatant of fermentation possessed best antimicrobial activity in all extracts whose MIC value is 31.2 μg/ml against T. rubrum, T. mentagrophytes, S. aureus and P. aeruginosa. And ZOI value is 29.01, 21.02, 35.02, 28.01 mm against T. rubrum, T. mentagrophytes, S. aureus and P. aeruginosa. Then we compare the puffball fermentation supernatant with blank contrast by LC-MS. There are the characteristic peaks named PBR-1 and PBR-2 with the puffball fermentation supernatant, the separation of compound PBR-1 and PBR-2 was done on semi-preparative C18 column and the MIC and ZOI of compound PBR-1 and PBR-2 are 15.6 μg/ml and 34 mm with the antifungal test. The fermentation supernatant and compound PBR-1 and PBR-2 have promising antifungal activity against T. rubrum and T. mentagrophytes.

References

Jan 1, 1988·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·E A Nunez
Aug 1, 1997·Current Opinion in Chemical Biology·Y Koltin, C A Hitchcock
Sep 29, 1999·Phytochemistry·V VajsS Milosavljević
Jan 12, 2000·Planta medica·C L CantrellN H Fischer
Dec 1, 2001·Biochemical and Biophysical Research Communications·Y W LamH X Wang
Jul 3, 2003·Fitoterapia·N A Awadh AliU Lindequist
Feb 24, 2004·Journal of Medicinal Food·P S NegiG K Jayaprakasha
May 10, 2006·Mycoses·Assya DjeridaneAomar Ammar-Khodja
Feb 9, 2008·Analytical and Bioanalytical Chemistry·Viktoria VukicsAndras Guttman
Apr 2, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Cornaglia
Apr 11, 2012·International Journal of Molecular Sciences·Rahele GhanbariNazamid Saari
Sep 3, 2013·Asian Pacific Journal of Tropical Biomedicine·Mohammad Amzad HossainQasim Al-Riyami
Dec 11, 2014·Journal of Colloid and Interface Science·Artur Schmidtchen, Martin Malmsten
Mar 31, 2015·Medicinal Research Reviews·Qi-Wei JiangZhi Shi
Dec 4, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Guo-Ying ZuoGen-Chun Wang

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.